利用干细胞组织工程技术进行继发性牙槽裂移植的放射学体积评估

Abdallah El Ali, Dina Abd-Alfattah, Khalid Ali
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引用次数: 0

摘要

目的:比较可吸收基质中扩增间充质干细胞(MSCs)与标准髂骨松质骨移植的牙槽裂骨移植的影像学结果。对象和方法:选取15例7 ~ 12岁单侧牙槽裂患者,随机分为两组:第一组:自体骨髓干细胞扩增后植入海绵胶原、富血小板血浆和纳米羟基磷灰石进行牙槽裂移植。第二组:前髂骨松质骨牙槽裂移植术。利用计算机辅助工程技术,从锥束CT上计算理想裂隙体积。术后3个月和6个月分别进行锥形束CT和骨填充的随访评估。结果:I组和II组的平均理想裂容分别为678 mm3和726 mm3,差异无统计学意义。术后3个月,I组和II组的平均骨填充体积(BF)有显著差异(分别为7% BF和47% BF)。此外,6个月时差异显著增加,第一组达到10%的BF,第二组达到60%的BF。结论:用于干细胞移植的胶原海绵、PRP和纳米羟基磷灰石混合支架在力学性能和生物降解性能方面仍有待改进,以支持骨组织工程。此外,作为金标准的松质骨移植物的放射学体积分析显示结果不理想,需要进一步研究以改善结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Radiographic Volumetric assessment of secondary alveolar cleft grafting using stem cells tissue-engineering
Objective: To compare the radiographic outcome of alveolar cleft bone grafting with expanded mesenchymal stem cells (MSCs) seeded into a resorbable matrix to standard iliac cancellous bone graft. Subjects and methods: Fifteen patients with unilateral alveolar cleft aged 7-12 years old were randomly divided into two groups: Group I: Alveolar cleft grafting with expanded autogenous bone marrow stem cells seeded in collagen sponge, platelet rich plasma and nanohydroxyapatite. Group II: Alveolar cleft grafting with cancellous bone harvested from anterior iliac crest. Ideal cleft volume was calculated from cone beam CT with computer aided engineering. Follow up evaluation with cone beam CT and Bone fill was calculated on three and six months postoperatively. Results: The mean ideal cleft volume was 678 mm3 and 726 mm3 in group I and group II respectively with no significant difference. At three months postoperatively, there was a Significantly difference in the mean volume of bone fill (BF) between group I and group II (7% BF and 47% BF respectively). Furthermore, the difference increased significantly at six months to become 10% BF in group I and 60% BF in group II. Conclusion: The hybridized scaffold used in the study for seeding of stem cells that contains collagen sponge, PRP and nanohydroxyapatite still needs improvement in mechanical and biodegradation properties to support bone tissue engineering. Moreover, the radiographic volumetric analysis of cancellous bone graft which is the gold standard revealed suboptimal results that needs further researches to improve the outcome.
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