C. Tao, Qian Guoliang, Yang Xiao-li, Ma Junyi, Hu Baishi, L. Fengquan
{"title":"一种蚁群感应信号分子的检测。瓜蒌及其对致病性的调节作用","authors":"C. Tao, Qian Guoliang, Yang Xiao-li, Ma Junyi, Hu Baishi, L. Fengquan","doi":"10.1017/S1479236209002514","DOIUrl":null,"url":null,"abstract":"An efficient AHL ( N -acyl-homoserine lactone) bioassay strain, JZA1, of Agrobacterium tumefaciens was used to detect the AHL production from Acidovorax avenae subsp. citrulli [the pathogen causing bacterial fruit blotch (BFB) of melons], and the results showed that A. avenae subsp. citrulli produced a 3-O-C 8 -homoserine (HSL) type signal molecule. Gene aiiA , which could degrade AHL molecules, was transformed into A. avenae subsp. citrulli strain NJF10, creating strain NJF10- aiiA . The AHL production from NJF10- aiiA was significantly reduced compared with wild-type NJF10. Inoculation tests showed that NJF10- aiiA had an obvious reduction of virulence on watermelon fruits. Our finds showed that AHL production by A. avenae subsp. citrulli was related to its pathogenicity. This work might provide a novel way to control BFB by QS (quorum sensing) interference.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"11","resultStr":"{\"title\":\"Detection of a quorum sensing signal molecule of Acidovorax avenae subsp. citrulli and its regulation of pathogenicity\",\"authors\":\"C. Tao, Qian Guoliang, Yang Xiao-li, Ma Junyi, Hu Baishi, L. Fengquan\",\"doi\":\"10.1017/S1479236209002514\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"An efficient AHL ( N -acyl-homoserine lactone) bioassay strain, JZA1, of Agrobacterium tumefaciens was used to detect the AHL production from Acidovorax avenae subsp. citrulli [the pathogen causing bacterial fruit blotch (BFB) of melons], and the results showed that A. avenae subsp. citrulli produced a 3-O-C 8 -homoserine (HSL) type signal molecule. Gene aiiA , which could degrade AHL molecules, was transformed into A. avenae subsp. citrulli strain NJF10, creating strain NJF10- aiiA . The AHL production from NJF10- aiiA was significantly reduced compared with wild-type NJF10. Inoculation tests showed that NJF10- aiiA had an obvious reduction of virulence on watermelon fruits. Our finds showed that AHL production by A. avenae subsp. citrulli was related to its pathogenicity. This work might provide a novel way to control BFB by QS (quorum sensing) interference.\",\"PeriodicalId\":236932,\"journal\":{\"name\":\"Chinese Journal of Agricultural Biotechnology\",\"volume\":\"5 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Journal of Agricultural Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1017/S1479236209002514\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Agricultural Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1017/S1479236209002514","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Detection of a quorum sensing signal molecule of Acidovorax avenae subsp. citrulli and its regulation of pathogenicity
An efficient AHL ( N -acyl-homoserine lactone) bioassay strain, JZA1, of Agrobacterium tumefaciens was used to detect the AHL production from Acidovorax avenae subsp. citrulli [the pathogen causing bacterial fruit blotch (BFB) of melons], and the results showed that A. avenae subsp. citrulli produced a 3-O-C 8 -homoserine (HSL) type signal molecule. Gene aiiA , which could degrade AHL molecules, was transformed into A. avenae subsp. citrulli strain NJF10, creating strain NJF10- aiiA . The AHL production from NJF10- aiiA was significantly reduced compared with wild-type NJF10. Inoculation tests showed that NJF10- aiiA had an obvious reduction of virulence on watermelon fruits. Our finds showed that AHL production by A. avenae subsp. citrulli was related to its pathogenicity. This work might provide a novel way to control BFB by QS (quorum sensing) interference.
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