球孢白僵菌规模化生产的研究Vuill。稻瘟病的防治:稻瘟病的防治

P. Das, L. K. Hazarika, D. Bora
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引用次数: 2

摘要

稻瘟病是全球作物生产的重要制约因素之一。它攻击夏稻的营养阶段,造成35-65%的损失,淹水后插秧水稻造成100%的产量损失,因此它被认为是阿萨姆邦水稻生产的主要限制因素之一。球孢白僵菌Vuill。是一种天然产生的强效昆虫病原真菌,已成功地用于控制水稻的多种害虫,包括稻瘟病。球孢白僵菌(Bals.)Vuill(菌株AAU-09)在马铃薯肉汤、米粥、椰子水、稻壳、锯末和米糠中大量培养。结果表明,在固体培养基中,添加2%葡萄糖的稻壳对棉蚜成虫的孢子产量最高(6.25 × 107孢子/ml),致病性最高(86.67%)。同样,在液体培养基中,添加合成几丁质(2%)、葡萄糖(2%)和蛋白胨(2%)的马铃薯肉汤支持最大的孢子产量。几丁质不仅对孢子的产生至关重要,而且对增加刺穿角质层的能力也至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study on Mass Production of Beauveria bassiana (Bals.) Vuill. for the Management of Rice Hispa, Dicladispa armigera (Olivier)
Rice hispa D. armigera is one of the most important constraints to crop production globally. It attacks the vegetative stages of summer rice causing 35-65% loss and post flooded transplanted rice causing 100% loss in yield throughout Assam and hence it is regarded as one of the major limiting factors in rice production in Assam. Beauveria bassiana (Bals.) Vuill. is a naturally occurring potent entomopathogenic fungus which has been successfully utilized for controlling many insect pests of rice including rice hispa. For developing a good formulation, Beauveria bassiana (Bals.) Vuill (Strain AAU-09) is mass cultured in the potato broth, rice gruel, coconut water, rice husk, sawdust and rice bran. It was seen that amongst the solid media ,rice husk in addition of 2% dextrose was the best in terms of spore production (6.25 x 107 conidia/ml) and pathogenecity (86.67%) to Dicladispa armigera (Olivier) (Coleoptera: Chrysomelidae) adults. Likewise, amongst the liquid media potato broth supplemented with synthetic chitin (2%), dextrose (2%) and peptone (2%) supported maximum spore production. Chitin is essential not only for spore production but also increasing the power of penetrating on cuticle.
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