Dual effect of epigallocatechine gallate on the pathology of Alzheimer s: Cholinesterases and amyloidogenesis

Aim: To evaluate the dual inhibitory effect of epigallocatechin gallate on Alzheimer’s pathogenesis. Methods: Cholinesterase inhibition studies were performed by kinetic Ellman methods and also the inhibitory effect of molecule on amyloid fibrillation is determined by dye binding thioflavin T method. Results: Epigallocatechin gallate inhibited both types of cholinesterases and amyloid fibrillation. The inhibition of acetylcholinesterase indicated an uncompetitive inhibition whereas butyrylcholinesterase inhibition had an unique pattern. Compound inhibited butyrylcholinesterase with two types of inhibition in a dose related manner. Fibrillation destabilization is also observed by the compound. Conclusion: Our results indicated that epigallocatechin gallate may be accepted as a candidate molecule as the dual effective drugs for neurodegenerative diseases.