Measurement of Interleukin-1β Level in Gingival Crevicular Fluid in Periodontitis Patients Treated by Non-Surgical Periodontal Therapy Combined with Topical Application of Melatonin Gel

Aim: The present study was performed to evaluate the efficacy of topical application of melatonin gel on clinical parameter and biochemical level of IL-1-β in gingival crevicular fluid in patients with periodontitis treated by non-surgical periodontal therapy. Subjects and methods: In this study, forty periodontitis patients with stage І, П grade A, were divided randomly into two groups: Group І: Twenty periodontitis patients with stage І, II grade A treated with conventional periodontal treatment (scaling and root planing) combined with intra pocket application of 2% melatonin gel. Group П: Twenty periodontitis patients with stage І,II grade A treated with conventional periodontal treatment alone (scaling and root planing). All patients were evaluated clinically at; base line and after 3 and 6 months. Also, biochemical evaluation of IL-1β using ELISSA at; base line and after 2 weeks, 1and 3 months. Results of the present study were recorded, tabulated and statistically analyzed. Results: clinical findings showed a statistically significant difference in all groups at the different intervals when compared to the baseline, biochemical evaluation of IL-1β was a statistically significant difference between group I and group II at 2 weeks and after 1 month only during the period of application. Conclusion: Adjunctive use of topically applied melatonin gel 2% appeared to be has beneficial effect on both clinical and biochemical parameters in patients with stage І, П, grade A periodontitis, with a statistically significant difference between group I and group II at 2 weeks and after 1 month of treatment (during the period of melatonin application) however, this effect is not significant when compared with scaling and root planning alone after 3 months of treatment