Renewable implementation of rational biomolecular systems design

Recently, a rationally designed method enabling the development of large-scale biomolecular circuits by combining three basic elements (catalysis, degradation, and annihilation mechanisms) like a Lego block was established. Although this method is instrumental in forming a functional biomolecular circuit, the circuit can operate normally only for a limited amount of time, which is a serious limitation in the field of DNA computing. In this study, we developed a conventional method of biomolecular implementation to make linear input-to-output systems renewable.