Detection of Toxoplasma Gondii DNA in Uterine and Malignant Prostate Tissues of Cancer Patients

The current study aimed to find the Toxoplasma gondii parasite in uterine and prostate tissue models infected with cancer, which were collected from the cities of Kut and Nasiriyah, and to identify infected organs using Real-time PCR based on (B1) gene and histological examination, and the other is to identify histological changes in tissues. Cancer as a result of infection with the parasite. By examining the histological sections of samples positive for the parasite, (120) hundred and twenty tumor tissue samples fixed with formalin as well as embedded with paraffin wax were collected from incoming patients and subjected to surgical intervention for samples located in the tissue laboratories of Al-Zahra and Al-Nasiriyah Teaching Hospital and previously diagnosed with prostate and uterine cancer a month ago October 2022 to January 2023 in my province of Wasit and Dhi Qar / Iraq, whose ages ranged from (25-93 years), as it included (40) samples of the prostate for males and (80) samples of uterine tissue from females. Positive samples were taken using real-time PCR technology, and tissue sections were prepared to diagnose parasite presence and to identify histological changes in infected tissue models. The total number of samples positive for T. gondii parasite, based on the tissue sections, was 53 (44.16%) distributed according to organs, the percentage of infection was recorded in the prostate 5 (35.71%) and the uterus 19 (48.71%). These results indicated the possible role of infection with the Toxoplasma gondii parasite in causing different types of infection cancer. It is concluded that infection with the Toxoplasma gondii parasite and its presence inside the cells has a role in the development of different types of histopathological changes, which in the future turn into possible cancers in the affected organs