低分子血清蛋白的肾毒性:肌红蛋白、血红蛋白、bence-jones蛋白和tamm-horsfall黏蛋白之间的理化相互作用。

D H Clyne, K S Kant, A J Pesce, V E Pollak
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引用次数: 0

摘要

三种低分子血清蛋白:肌红蛋白、血红蛋白和BENCE-JONES蛋白在临床上与急性肾功能衰竭相关。它们都有等电点,使它们在血液pH值下呈阴离子,但在酸尿条件下远端肾元呈阳离子。将这些蛋白与TAMM-HORSFALL粘蛋白在体外混合,并用lN HCl降低pH值的实验显示,在pH为5.5及以下时,这些蛋白会共沉淀。在体内实验中,将11种不同的BENCE-JONES蛋白(pl值在5.2 ~ 6.6之间)注射到酸尿酸中,当BENCE-JONES蛋白的pl值大于5.7时,缺水大鼠血清尿素氮和肌酐浓度急剧升高,而在BENCE-JONES蛋白的pl值小于5.7时,大鼠的血清尿素氮和肌酐浓度变化不大。这些数据表明蛋白质pl和尿液pH值在确定肾毒性方面是重要的;这些低分子量血清蛋白和TAMM-HORSFALL蛋白在远端肾元中相互作用引发急性肾功能衰竭的机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nephrotoxicity of low molecular weight serum proteins: physicochemical interactions between myoglobin, hemoglobin, bence-jones proteins and tamm-horsfall mucoprotein.

Three types of low molecular weight serum proteins, myoglobin, hemoglobin and BENCE-JONES proteins, are associated clinically with acute renal failure. All have isoelectric points which render them anionic at blood pH but cationic in the distal nephron under conditions of aciduria. Experiments in which these proteins were mixed with TAMM-HORSFALL mucoprotein in vitro and the pH lowered with lN HCl showed co-precipitation of proteins at pH levels of 5.5 and below. In vivo experiments in which 11 different BENCE-JONES proteins of pl ranging from 5.2 to 6.6 were injected into aciduric, hydropenic rats showed an acute rise in serum urea nitrogen and creatinine concentrations with BENCE-JONES proteins of pl greater than 5.7 compared with little change in rats injected with BENCE-JONES proteins of pl less than 5.7. These data suggest that protein pl and urine pH are important in determining nephrotoxicity; a mechanism by which these low molecular weight serum proteins and TAMM-HORSFALL proteins interact in the distal nephron to initiate acute renal failure in postulated.

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