流式细胞仪用于血液细胞浓度的参考测量，不确定度低

2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA) Proceedings Pub Date : 2015-05-07 DOI:10.1109/MeMeA.2015.7145258

M. Kammel, A. Kummrow, M. John, S. Reitz, K. Witt, J. Neukammer

{"title":"流式细胞仪用于血液细胞浓度的参考测量，不确定度低","authors":"M. Kammel, A. Kummrow, M. John, S. Reitz, K. Witt, J. Neukammer","doi":"10.1109/MeMeA.2015.7145258","DOIUrl":null,"url":null,"abstract":"A method is described to determine cell concentrations for erythrocytes and leukocytes with typical uncertainties of 0.8% and 2%, respectively. The unique method is designed in such a way that results are traceable to SI derived units. The sample suspensions are prepared under gravimetric control. The sample volume measured in cell counting is determined by weighing, including separate density measurements. Random coincidences are a significant challenge in particle counting leading to underestimated cell concentrations. In the method described here this challenge is met by recording the integrated dead time in particle counting and applying dilution series. This method is now routinely applied to provide reference measurement values for external quality assurance of German hematology laboratories for the complete blood count.","PeriodicalId":277757,"journal":{"name":"2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA) Proceedings","volume":"50 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2015-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Flow cytometer for reference measurements of blood cell concentrations with low uncertainty\",\"authors\":\"M. Kammel, A. Kummrow, M. John, S. Reitz, K. Witt, J. Neukammer\",\"doi\":\"10.1109/MeMeA.2015.7145258\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A method is described to determine cell concentrations for erythrocytes and leukocytes with typical uncertainties of 0.8% and 2%, respectively. The unique method is designed in such a way that results are traceable to SI derived units. The sample suspensions are prepared under gravimetric control. The sample volume measured in cell counting is determined by weighing, including separate density measurements. Random coincidences are a significant challenge in particle counting leading to underestimated cell concentrations. In the method described here this challenge is met by recording the integrated dead time in particle counting and applying dilution series. This method is now routinely applied to provide reference measurement values for external quality assurance of German hematology laboratories for the complete blood count.\",\"PeriodicalId\":277757,\"journal\":{\"name\":\"2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA) Proceedings\",\"volume\":\"50 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-05-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA) Proceedings\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/MeMeA.2015.7145258\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA) Proceedings","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/MeMeA.2015.7145258","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 5

摘要

本文描述了一种测定红细胞和白细胞浓度的方法，其典型不确定度分别为0.8%和2%。独特的方法是这样设计的，结果可追溯到SI派生单位。样品悬浮液是在重量控制下制备的。在细胞计数中测量的样品体积是通过称重确定的，包括单独的密度测量。随机巧合是粒子计数中的一个重大挑战，导致细胞浓度被低估。在这里描述的方法中，通过记录颗粒计数中的积分死区时间和应用稀释级数来满足这一挑战。该方法现已常规应用于德国血液学实验室全血细胞计数的外部质量保证提供参考测量值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Flow cytometer for reference measurements of blood cell concentrations with low uncertainty

A method is described to determine cell concentrations for erythrocytes and leukocytes with typical uncertainties of 0.8% and 2%, respectively. The unique method is designed in such a way that results are traceable to SI derived units. The sample suspensions are prepared under gravimetric control. The sample volume measured in cell counting is determined by weighing, including separate density measurements. Random coincidences are a significant challenge in particle counting leading to underestimated cell concentrations. In the method described here this challenge is met by recording the integrated dead time in particle counting and applying dilution series. This method is now routinely applied to provide reference measurement values for external quality assurance of German hematology laboratories for the complete blood count.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA) Proceedings

自引率

0.00%

发文量