日惹市畅销休闲食品中大肠菌群及肠道致病菌的检测

T. Y. Budiarso, C. Amarantini, G. Prihatmo, R. Restiani, Yesika Putri, Virgin Kindagen, Sharoneva Linggardjati
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引用次数: 0

摘要

日惹居民最喜欢的小吃很受欢迎,比如泡菜、串肉丸和饺子。这些食品的加工和服务过程不注意卫生方面,因此,有必要监测大肠菌群和肠道病原体的存在或不存在,这些细菌经常导致消化系统紊乱。从不同地点的每种食物中共收集了30个样本。然后将这些样品在CCA培养基上进行枚举,以培养所有类型的大肠菌群和肠道病原体。然后在SSA, SMAC和DFI琼脂培养基上选择所得菌落,获得单个分离物,进行生化测试,直到使用API 20E鉴定其属水平。根据30份食品样品的鉴定结果,得到的污染水平分别为大肠埃希菌(16.6%)、肺炎克雷伯菌(13.3%)、小肠结肠炎耶尔森菌(13.3%)、Pantoea spp(6.6%)。、嗜水气单胞菌(6.6%)、阴沟肠杆菌(6.6%)、粘质沙雷氏菌(6.6%)、博德氏菌/阿尔卡利菌/莫拉氏菌(6.6%)、液化沙雷氏菌(3.3%)、奇异变形杆菌(3.3%)、志贺氏菌(3.3%)、美洲埃温氏菌(3.3%)。基于这些发现,有必要对日惹市的街头食品持谨慎态度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of Coliforms and Enteric Pathogens in Favorite Snack Food Sold in Yogyakarta City
Favorite snack food is very popular to Yogyakarta's residents, such as cilok, skewered meatballs, and dumplings. The processing and serving processes of these food does not pay attention to hygiene aspects, therefore, it is necessary to monitor the presence or absence of coliform bacteria and enteric pathogens that often cause digestive disorders. A total of 30 samples were collected from each food from different locations. These samples were then enumerated on a CCA medium to grow all types of coliforms and enteric pathogens. The resulted colonies were then selected on SSA, SMAC, and DFI Agar medium to obtain a single isolate, which were biochemically tested until their genus levels were identified using API 20E. Based on the identification results of 30 food samples, the contamination levels obtained were as follows, Escherichia coli (16.6%), Klebsiella pneumoniae (13.3%), Yersinia enterocolitica (13.3%), Pantoea spp (6.6%). ), Aeromonas hydrophila (6.6%), Enterobacter cloacae (6.6%), Serratia marcescens (6.6%), Bordetella / Alcaligenes / Moraxella spp (6.6%), Serratia liquefaciens (3.3%), Proteus mirabilis (3.3%), Shigella spp (3.3%), and Ewingella Americana (3.3%). Based on these findings, it is necessary to be cautious of street food in Yogyakarta City.
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