T. Y. Budiarso, C. Amarantini, G. Prihatmo, R. Restiani, Yesika Putri, Virgin Kindagen, Sharoneva Linggardjati
{"title":"日惹市畅销休闲食品中大肠菌群及肠道致病菌的检测","authors":"T. Y. Budiarso, C. Amarantini, G. Prihatmo, R. Restiani, Yesika Putri, Virgin Kindagen, Sharoneva Linggardjati","doi":"10.2991/ASSEHR.K.210305.007","DOIUrl":null,"url":null,"abstract":"Favorite snack food is very popular to Yogyakarta's residents, such as cilok, skewered meatballs, and dumplings. The processing and serving processes of these food does not pay attention to hygiene aspects, therefore, it is necessary to monitor the presence or absence of coliform bacteria and enteric pathogens that often cause digestive disorders. A total of 30 samples were collected from each food from different locations. These samples were then enumerated on a CCA medium to grow all types of coliforms and enteric pathogens. The resulted colonies were then selected on SSA, SMAC, and DFI Agar medium to obtain a single isolate, which were biochemically tested until their genus levels were identified using API 20E. Based on the identification results of 30 food samples, the contamination levels obtained were as follows, Escherichia coli (16.6%), Klebsiella pneumoniae (13.3%), Yersinia enterocolitica (13.3%), Pantoea spp (6.6%). ), Aeromonas hydrophila (6.6%), Enterobacter cloacae (6.6%), Serratia marcescens (6.6%), Bordetella / Alcaligenes / Moraxella spp (6.6%), Serratia liquefaciens (3.3%), Proteus mirabilis (3.3%), Shigella spp (3.3%), and Ewingella Americana (3.3%). Based on these findings, it is necessary to be cautious of street food in Yogyakarta City.","PeriodicalId":378773,"journal":{"name":"Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020)","volume":"13 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of Coliforms and Enteric Pathogens in Favorite Snack Food Sold in Yogyakarta City\",\"authors\":\"T. Y. Budiarso, C. Amarantini, G. Prihatmo, R. Restiani, Yesika Putri, Virgin Kindagen, Sharoneva Linggardjati\",\"doi\":\"10.2991/ASSEHR.K.210305.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Favorite snack food is very popular to Yogyakarta's residents, such as cilok, skewered meatballs, and dumplings. The processing and serving processes of these food does not pay attention to hygiene aspects, therefore, it is necessary to monitor the presence or absence of coliform bacteria and enteric pathogens that often cause digestive disorders. A total of 30 samples were collected from each food from different locations. These samples were then enumerated on a CCA medium to grow all types of coliforms and enteric pathogens. The resulted colonies were then selected on SSA, SMAC, and DFI Agar medium to obtain a single isolate, which were biochemically tested until their genus levels were identified using API 20E. Based on the identification results of 30 food samples, the contamination levels obtained were as follows, Escherichia coli (16.6%), Klebsiella pneumoniae (13.3%), Yersinia enterocolitica (13.3%), Pantoea spp (6.6%). ), Aeromonas hydrophila (6.6%), Enterobacter cloacae (6.6%), Serratia marcescens (6.6%), Bordetella / Alcaligenes / Moraxella spp (6.6%), Serratia liquefaciens (3.3%), Proteus mirabilis (3.3%), Shigella spp (3.3%), and Ewingella Americana (3.3%). Based on these findings, it is necessary to be cautious of street food in Yogyakarta City.\",\"PeriodicalId\":378773,\"journal\":{\"name\":\"Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020)\",\"volume\":\"13 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-03-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2991/ASSEHR.K.210305.007\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2991/ASSEHR.K.210305.007","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Detection of Coliforms and Enteric Pathogens in Favorite Snack Food Sold in Yogyakarta City
Favorite snack food is very popular to Yogyakarta's residents, such as cilok, skewered meatballs, and dumplings. The processing and serving processes of these food does not pay attention to hygiene aspects, therefore, it is necessary to monitor the presence or absence of coliform bacteria and enteric pathogens that often cause digestive disorders. A total of 30 samples were collected from each food from different locations. These samples were then enumerated on a CCA medium to grow all types of coliforms and enteric pathogens. The resulted colonies were then selected on SSA, SMAC, and DFI Agar medium to obtain a single isolate, which were biochemically tested until their genus levels were identified using API 20E. Based on the identification results of 30 food samples, the contamination levels obtained were as follows, Escherichia coli (16.6%), Klebsiella pneumoniae (13.3%), Yersinia enterocolitica (13.3%), Pantoea spp (6.6%). ), Aeromonas hydrophila (6.6%), Enterobacter cloacae (6.6%), Serratia marcescens (6.6%), Bordetella / Alcaligenes / Moraxella spp (6.6%), Serratia liquefaciens (3.3%), Proteus mirabilis (3.3%), Shigella spp (3.3%), and Ewingella Americana (3.3%). Based on these findings, it is necessary to be cautious of street food in Yogyakarta City.