血浆β -连环蛋白检测:慢性乙型肝炎患者肝纤维化程度评估

Anne Marrya, B. Sidharta, Lusi Oka Wardhani, J. Suparyatmo, M. Pramudianti
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引用次数: 0

摘要

慢性乙型肝炎感染引起的肝纤维化导致显著的死亡率和发病率。肝纤维化进展的监测和评估取决于检测纤维化的能力。肝活检作为肝纤维化的金标准是一种侵入性技术,而纤维扫描与瞬态弹性成像作为一种非侵入性技术具有局限性。因此,需要一种生物标志物来检测肝纤维化。β -连环蛋白是一种多功能蛋白,具有wnt调控的转录因子,存在于肝细胞中。乙型肝炎病毒感染激活Wnt/ β -连环蛋白,影响肝纤维化靶基因的表达。本研究旨在分析血浆β -连环蛋白水平作为评估慢性乙型肝炎感染患者肝纤维化程度的标准参考。这是一项采用横断面设计的观察性分析研究。该分析是在2020年12月至2021年1月期间对苏拉arta Dr. Moewardi医院的70名慢性乙型肝炎患者进行的。本ROC分析用于确定分界点。采用2x2诊断试验台选择最佳AUC点。血浆β -连环蛋白的临界值为73.132 pg/mL, AUC为0.793 (CI 95%: 0.681-0.906;p8 kPa。血浆β -连环蛋白水平作为肝纤维化标志物表现中等。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Beta-Catenin Plasma Test: Liver Fibrosis Degree Assessment in Chronic Hepatitis B Patients
Liver fibrosis caused by chronic hepatitis B infection leads to significant mortality and morbidity. Monitoring and evaluation of liver fibrosis progression depend on the ability to detect the fibrosis. Liver biopsy as a gold standard for liver fibrosis is an invasive technique, while Fibroscan ® with transient elastography as a non-invasive technique has a limitation. Therefore, a biomarker is needed to detect liver fibrosis. Beta-catenin is a multifunctional protein, which has a Wnt-regulated transcription factor and resides in hepatocytes. Hepatitis B virus infection activates Wnt/beta-catenin and affects the expression of target genes for liver fibrosis. This study aimed to analyze the diagnostic performance of plasma beta-catenin levels using transient elastography as a standard reference to assess the degree of liver fibrosis in patients with chronic hepatitis B infection. This was an observational analytic study with a cross-sectional design. The analysis was performed on 70 chronic Hepatitis B patients between December 2020 and January 2021 at Dr. Moewardi Hospital, Surakarta. This ROC analysis was used to determine the cut-off point. The best AUC point was chosen using a 2x2 diagnostic test table. The cut-off point for plasma beta-catenin was 73.132 pg/mL and AUC was 0.793 (CI 95%: 0.681-0.906; p<0.001), indicating that the results were statistically significant with p<0.05. Sensitivity of 74.3%; specificity of 71.4%; PPV of 72.2%; NPV of 73.5%; LR (+) 2.6; LR (-) 0.36 were obtained. The beta-catenin level was <73.132 pg/mL. A total of 26 subjects were at risk for liver fibrosis with transient elastography >8 kPa. Plasma beta-catenin levels had moderate performance as a liver fibrosis marker.
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