菠萝蛋白酶粗提物促进辣椒幼苗出苗和生长

Lianny Pérez-Gómez, Y. Acosta, Evelio Báez, C. Linares, C. Carvajal, S. Naidoo, Aurora Pérez-Martínez
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引用次数: 0

摘要

辣椒(Capsicum annuum L.)是世界上消费最多的园艺产品之一。然而,辣椒种子萌发缓慢且不同步。有机农业鼓励使用天然物质对种子进行发芽前处理。本研究的目的是评价用菠萝蛋白酶粗提物(从菠萝茎渣中提取)对甜椒种子随后的出苗和生长的影响。考察了浸提液蛋白水解活性(0、3.16、6.25、12.25和25.0 totalU)、吸胀时间(0、3、6、9、12和15 h)和吸胀温度(15、25、35和45℃)对幼苗出苗和生长的影响。以羽化率E、羽化至50%时间TE50、羽化指数EI、平均羽化率MER、平均羽化时间MET和同步指数SI为指标,在35℃、总u为6.25的凤梨蛋白酶粗提物处理3 h时,羽化效果显著最佳。经过这种组合处理的种子也产生了最旺盛的幼苗(真叶数量、茎的长度和厚度、新鲜质量、干质量、叶面积)。综上所述,在35℃条件下,用总u蛋白水解活性为6.25的菠萝蛋白酶粗提物预处理3 h,可促进甜椒幼苗出苗和生长。这一结果为甜椒种子提供了一种有机启动替代方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bromelain crude extract to improve the emergence and growth of pepper seedlings
Pepper (Capsicum annuum L.) is one of the most consumed horticultural products worldwide. However, pepper seeds have slow and asynchronous germination. Organic agriculture encourages using natural substances for the pre-germinative treatment of seeds. The objective of the present work was to evaluate the effect of priming sweet pepper seeds with bromelain crude extract (obtained from pineapple stem residues) on subsequent seedling emergence and growth. The effects of the proteolytic activity of the extract (0, 3.16, 6.25, 12.25, and 25.0 totalU), imbibition time (0, 3, 6, 9, 12, and 15 h), and imbibition temperature (15, 25, 35 and 45 ℃) on seedling emergence and growth were assessed. The significantly best emergence, based on a range of indicators (emergence percentage: E, time to 50% emergence: TE50, emergence index: EI, mean emergence rate: MER, mean emergence time: MET, and synchronization index: SI), was obtained when the seeds were treated for three h at 35°C with a 6.25 totalU bromelain crude extract. Seeds subjected to this treatment combination also yielded the most vigorous seedlings (number of true leaves, length and thickness of the stems, fresh mass, dry mass, leaf area) . In conclusion, pre-germinative treatment with bromelain crude extract of 6.25 totalU proteolytic activity for three h at 35°C improved seedling emergence and growth in sweet pepper seedlings relative to untreated seed. This result offers an organic priming alternative for seeds of sweet pepper.
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