巴拉圭克氏锥虫分离株随机扩增多态性DNA的比较研究

Japanese Journal of Tropical Medicine and Hygiene Pub Date : 1999-09-15 DOI:10.2149/TMH1973.27.427

S. Baba, Tamami Matsumoto, H. Kanbara, M. Sakamoto, M. Maldonado, A. R. Arias, H. Saya, T. Mimori

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引用次数: 2

摘要

基于随机扩增多态性DNA (RAPD)序列的Dice相似系数矩阵，采用非加权对群分析法(UPGMA)对巴拉圭克氏锥虫分离株进行变异分析。还将这些分离株与从南美洲巴西、智利和哥伦比亚分离的已建立的菌株进行了比较。在9株巴拉圭菌株中，3株与Berenice、Y和Sao Felipe菌株相似(酵母菌型;巴西产Z2)和4株与智利产Tulahuen菌株相似。在哥伦比亚未发现与哥伦比亚I型菌株(Z1)相似的分离株。(860 - 0811 ~~~;~~~:~~ 2 - 2 -我~ ~ ~,)f ~ ~ ~ ~ ~ ~ ~ ~ H ~ ~ ~ C ~ 1 ~ ~ - ~ ~我~ ~ ~ ~ ~ ~ ~;~ {~ ~)

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COMPARATIVE STUDY OF RANDOM AMPLIFIED POLYMORPHIC DNA OF TRYPANOSOMA CRUZI ISOLATES FROM PARAGUAY

The variation of Paraguayan Trypanosoma cruzi isolates was examined using the unweighted pair group method analysis (UPGMA) based on the resulting matrix of the Dice sirnilarity coefficients of random amplified polymorphic DNA (RAPD) profiles. These isolates were also compared with the established strains isolated from Brazil, Chile and Colombia in South America. Of 9 Paraguayan isolates, 3 isolates were similar to the Berenice, Y and Sao Felipe strains (zymodeme type; Brazilian Z2) , respectively, isolated frorn Brazil, and 4 strains were sirnilar to the Tulahuen strain from Chile. There was no isolate similar to the Colombiana I strain (Z1) from Colombia. (860-0811 ~~~;~~~:~~ 2 - 2 - I ~~~;)~~f~'~,~~~~H~~~~1~~-~C~i~ ~~~~~~; ~{~~)

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Japanese Journal of Tropical Medicine and Hygiene

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