低聚果糖培养基培养植物乳杆菌AKK-30抗致病菌活性的研究

Nisa Grendpina, D. Fitri, H. Julendra, A. Sofyan, E. Damayanti
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摘要

本研究的目的是评价植物乳杆菌AKK-30培养时间与低聚果糖(FOS)浓度的关系,以及植物乳杆菌AKK-30代谢产物对病原菌的抑菌作用。植物乳杆菌AKK-30是从土鸡小肠中分离得到的。L. plantarum AKK-30在含FOS(0%、0.5%、1%和1.5%)的MRSB培养基上培养,在37℃下孵育6、12、18、24小时。研究了植物乳杆菌AKK-30代谢物对大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌和白痢沙门氏菌4种病原菌的抑菌活性。结果表明,1% FOS浓度和24 h孵育对植物乳杆菌AKK-30生长最有效(2.11 × 108 CFU/ml)。植物L. plantarum AKK-30代谢产物抑菌活性提取物能够抑制大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和白蜡葡萄球菌的生长。对金黄色葡萄球菌的抑菌率最高,为10.8 mm,其次为大肠杆菌(9.9 mm)、白蜡葡萄球菌(9.083 mm)和铜绿假单胞菌(8.783 mm)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancing Anti-pathogenic Bacteria Activity of Lactobacillus Plantarum AKK-30 Cultured on the Medium Containing Fructose-Oligosaccharides
The purpose of this study was to evaluate the concentration of Fructose-Oligosaccharides (FOS) in correlation with incubation time for growing Lactobacillus plantarum AKK-30, and to assess metabolites of L. plantarum AKK-30 as antimicrobial substances against to pathogenic bacteria. L. plantarum AKK-30 was isolated from the small intestine of native chicken. L. plantarum AKK-30 was grown on MRSB medium containing FOS (0%, 0.5%, 1%, and 1.5%), and incubated at different times (6, 12, 18, and 24 hours) at 37°C. Antimicrobial activity of L. plantarum AKK-30 metabolites was tested on four species of pathogenic bacteria consisted of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella pullorum. The results showed that the concentration of 1% FOS and 24-hours incubation were most effective in increasing L. plantarum AKK-30 growth (2.11 x 108 CFU/ml). Antimicrobial activity extract of L. plantarum AKK-30 metabolites was able to inhibit the growth of E. coli, P. aeruginosa, S. aureus, and S. pullorum. The highest inhibition of bacteria was observed on S. aureus which was 10.8 mm, followed by E. coli at 9.9 mm, S. pullorum at 9.083 mm, and P. aeruginosa 8.783 mm.
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