Analysis of tissue specificity of alternative polyadenylation sites

The study of gene expression has become an effective way to explore complex life phenomena, and messenger RNA polyadenylation is a very important step in the process of eukaryotic gene expression. In this session, there is an important biological process called polyadenylation, in which polyadenylation sites (poly(A) sites) are formed, which usually mark the end of the transcript and the end of the gene. Some genes have multiple poly(A) sites. In this case, we call these polyadenylation sites Alternative polyadenylation sites (APA sites). APA sites play an important role in the study of eukaryotic transcription process and biological complexity. APA sites are used differently in different tissues, and the analysis of tissue specificity can also help us further explore biological functions. Therefore, this paper mainly conducts tissue-specific analysis on APA sites, obtains tissue-specific sites through information entropy and generalized linear mixed effect models, and further analyzes these sites. We have effectively screened tissue-specific APA sites through the method in this paper, and these APA sites are of great help to the study of polyadenylation.