CuraSelect:为基因特异性靶点挖掘复杂cDNA文库

Proceedings of the IEEE 27th Annual Northeast Bioengineering Conference (Cat. No.01CH37201) Pub Date : 2001-03-31 DOI:10.1109/NEBC.2001.924691

M. P. Daley, S. Colman, M. Popp, J. Simons

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引用次数: 0

摘要

为了加快基因鉴定和表征的进程，一种快速的基因分离方法是必不可少的。一种方法是从质粒文库中分离cDNA克隆。这里描述的是一个称为CuraSelect的过程，它使用少量已知序列数据从一个未知基因中靶向并特异性地从一个复杂的cDNA质粒文库中恢复它。在这种快速分离过程之后，感兴趣的基因可用于测序和进一步的表征研究。

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CuraSelect: mining complex cDNA libraries for gene specific targets

In order to accelerate the process of identifying and characterizing genes, a rapid method for gene isolation is essential. One approach is to isolate cDNA clones from a plasmid library. Described here is a process referred to as CuraSelect which uses a small amount of known sequence data from an otherwise unknown gene to target and specifically recover it from a complex cDNA plasmid library. Following this rapid isolation process, the gene of interest is available for sequencing and further characterization studies.

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Proceedings of the IEEE 27th Annual Northeast Bioengineering Conference (Cat. No.01CH37201)

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