{"title":"胃和食管活检常规特殊染色的使用","authors":"O. Belkacem","doi":"10.53555/eijmhs.v4i1.29","DOIUrl":null,"url":null,"abstract":"Problem: Special stains such as the Giemsa, the Alcian blue (AB) and the Periodic Acid Schiff (PAS) are widely used for gastric and/or esophageal biopsies for the diagnosis of Helicobacter pylori (H. pylori) and intestinal metaplasia (IM). The purpose of our study was to determine if these stains are actually needful \nMethods: We retrospectively studied 209 gastric and esophageal biopsies. We evaluated the H. pylori status on hematoxylin and eosin (H&E) stained slides and the presence of IM and finally we examined the special stains. \nResults: 23% of cases were H. pylori-positive. H&E stain had a high degree of accuracy (92,1%). The Giemsa stain was positive in 23,7% and negative in 71,8%. The Giemsa was useful in 16,7% biopsies. The AB revealed goblet cells in 4,8% cases. AB sensibility was 90,9%. From the 208 slides stained with PAS, 4,3% were classed IM-positive. PAS sensibility was 90%. The two special stains (AB and PAS) have made no diagnostic gain. \nConclusions: Routine special stains for every single gastric and/or esophageal biopsy are not required and H&E assessment combined with selective ordering of these stains will identify all cases of H. pylori gastritis and IM.","PeriodicalId":122699,"journal":{"name":"EPH - International Journal of Medical and Health Science","volume":"202 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"USE OF ROUTINE SPECIAL STAINS FOR GASTRIC AND ESOPHAGEAL BIOPSIES\",\"authors\":\"O. Belkacem\",\"doi\":\"10.53555/eijmhs.v4i1.29\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Problem: Special stains such as the Giemsa, the Alcian blue (AB) and the Periodic Acid Schiff (PAS) are widely used for gastric and/or esophageal biopsies for the diagnosis of Helicobacter pylori (H. pylori) and intestinal metaplasia (IM). The purpose of our study was to determine if these stains are actually needful \\nMethods: We retrospectively studied 209 gastric and esophageal biopsies. We evaluated the H. pylori status on hematoxylin and eosin (H&E) stained slides and the presence of IM and finally we examined the special stains. \\nResults: 23% of cases were H. pylori-positive. H&E stain had a high degree of accuracy (92,1%). The Giemsa stain was positive in 23,7% and negative in 71,8%. The Giemsa was useful in 16,7% biopsies. The AB revealed goblet cells in 4,8% cases. AB sensibility was 90,9%. From the 208 slides stained with PAS, 4,3% were classed IM-positive. PAS sensibility was 90%. The two special stains (AB and PAS) have made no diagnostic gain. \\nConclusions: Routine special stains for every single gastric and/or esophageal biopsy are not required and H&E assessment combined with selective ordering of these stains will identify all cases of H. pylori gastritis and IM.\",\"PeriodicalId\":122699,\"journal\":{\"name\":\"EPH - International Journal of Medical and Health Science\",\"volume\":\"202 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"EPH - International Journal of Medical and Health Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.53555/eijmhs.v4i1.29\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"EPH - International Journal of Medical and Health Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.53555/eijmhs.v4i1.29","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
USE OF ROUTINE SPECIAL STAINS FOR GASTRIC AND ESOPHAGEAL BIOPSIES
Problem: Special stains such as the Giemsa, the Alcian blue (AB) and the Periodic Acid Schiff (PAS) are widely used for gastric and/or esophageal biopsies for the diagnosis of Helicobacter pylori (H. pylori) and intestinal metaplasia (IM). The purpose of our study was to determine if these stains are actually needful
Methods: We retrospectively studied 209 gastric and esophageal biopsies. We evaluated the H. pylori status on hematoxylin and eosin (H&E) stained slides and the presence of IM and finally we examined the special stains.
Results: 23% of cases were H. pylori-positive. H&E stain had a high degree of accuracy (92,1%). The Giemsa stain was positive in 23,7% and negative in 71,8%. The Giemsa was useful in 16,7% biopsies. The AB revealed goblet cells in 4,8% cases. AB sensibility was 90,9%. From the 208 slides stained with PAS, 4,3% were classed IM-positive. PAS sensibility was 90%. The two special stains (AB and PAS) have made no diagnostic gain.
Conclusions: Routine special stains for every single gastric and/or esophageal biopsy are not required and H&E assessment combined with selective ordering of these stains will identify all cases of H. pylori gastritis and IM.