Evaluation of the Anti-adenoviral Activity of Ozonated Non-alcohol Disinfectant

Human mastadenovirus (HAdV) is highly resistant to common hand disinfectants. Epidemic keratoconjunctivitis and pharyngoconjunctival fever cause explosive infections in confined spaces, leading to nosocomial infections and other mass infections. Hemorrhagic cystitis and DIC caused by HAdV is also a major problem during hematopoietic stem cell transplantation, and since there are currently no effective antiviral agents against HAdV infection, it is important to control the spread of infection using disinfectants. The disinfecting and virucidal abilities of ozone are already well known. In 2019, we reported that ozonated alcohol agent has high virucidal potential. Contrarily, alcohol disinfectant is not a disinfectant that can be used universally; there are cases in which it cannot be used due to relig-ious beliefs. Therefore, in this study, we evaluated the antiviral activity of the agent with ozone stabilized in oil using adenovirus to evaluate its ability as a disinfectant. The viability of the virus was evaluated through reaction times ranging from 30 s to 5 min. As a result of this study, slight differences in reactivity were observed among HAdV types, but more than 70% of the virus was killed within 30 s, and the virus survival rate was reduced to 1/100 within 3 min. It was con-firmed that the ozonated oil disinfectant is capable of killing HAdVs.