细胞诱捕技术在生物细胞鉴定和检测中的应用

Sameh Sherif, Y. Ghallab, M. El-Wakad, Y. Ismail
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引用次数: 0

摘要

Dielectrophoresis (DEP)力已被确立为最强大的技术之一,可用于控制不同生物细胞中使用不同的技术,例如细胞分选,细胞转运和细胞捕获。如何改进和提升传感和检测阶段,提升整体检测方法,是必须达到的主要目标和挑战。在本研究中,我们建立了一种基于介电电泳响应和电阻抗检测相结合的新方法,这种结合可以实现细胞检测技术发展的主要目标。在不同的力分布下,介质电泳反应对细胞捕获有显著的影响。力的分布和细胞的响应可以结合起来,增强生物细胞和不同颗粒的识别和检测。以往和传统的细胞检测研究在进行介电参数检测和测量时,忽略了细胞分布对感测电极的影响,如在进行细胞阻抗检测时忽略了细胞分布。电池在感应电极上的随机分布是分析电池介电参数的一个重要因素。因此,我们关注的是利用电泳力的作用来克服细胞的随机分布,使细胞集中在传感电极上。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cell Trapping by Dielectrophoresis to Enhance the Identification and Detection of Biological Cell
Dielectrophoresis (DEP) force has established to be one of the strongest techniques can be used to control in the different biological cell using different techniques for example Cell sorting, Cell Transporting and Cell Trapping. The major target and challenge must be achieved are that how can improve and enhance the sensing and detection stage to enhance the overall detection method. In this study, we establish a new methodology based on the combination between the dielectrophoresis response and the electric impedance detection this combination can achieve the main target of the develop the cell detection techniques. The dielectrophoresis response can be extracted as a significant effect on the cell trapping under different force distribution. The force distribution and the cell response can be combined to enhance the biological cell and different particles identification and detection. The previous and the conventional studies on the cell detection ignore the effect of the cell distribution on the sensing and measuring electrodes during the dielectric parameters detection and measurements such as the ignoring of the cell distribution during the cell impedance detection. The random spread of the cells on the sensing electrodes consider a vital factor during cell dielectric parameters analysis. So, we focus on the using of the effect dielectrophoresis force can overcome this random cell distribution and make the cells being focused on the sensing electrodes.
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