模拟DIC显微镜图像:从物理原理到计算模型

Proceedings Workshop on Photometric Modeling for Computer Vision and Graphics (Cat. No.PR00271) Pub Date : 1999-06-22 DOI:10.1109/PMCVG.1999.787761

F. Kagalwala, T. Kanade

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引用次数: 4

摘要

差示干涉对比显微镜(DIC)是研究活生物细胞的一种强大的可视化工具。然而，它在定量分析中的应用受到图像与物体之间非线性关系的限制。结合图形学和物理学的概念，我们使用广义射线追踪器对这些非线性进行建模。我们通过比较制造标本的真实图像数据和虚拟物体的模拟图像来验证我们的模型。我们计划使用该模型迭代重建未知标本的三维特性。

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Simulating DIC microscope images: from physical principles to a computational model

Differential Interference Contrast (DIC) microscopy is a powerful visualization tool to study live biological cells. Its use in quantitative analysis, however, is limited by the nonlinear relation between image and object. Combining concepts from graphics and physics, we model these nonlinearities using a generalized ray tracer. We verify our model by comparing real image data of manufactured specimens to simulated images of virtual objects. We plan to use this model to iteratively reconstruct the three-dimensional properties of unknown specimens.

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Proceedings Workshop on Photometric Modeling for Computer Vision and Graphics (Cat. No.PR00271)

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