SARS-CoV-2感染检测现状及新方法

M. Mohammadi, A. Omidi, Hoda Sabati
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引用次数: 5

摘要

SARS-CoV-2是全球大流行的病原体,也被称为Covid-19。这种病毒属于一组冠状病毒,已影响全球1000多万人,造成全球近50万人死亡。新冠肺炎疫情已在全球蔓延,于2019年发源于中国湖北省武汉市。这种疾病是一个重大挑战,因为没有抗病毒治疗方法。本次审查将讨论当前实验室中检测SARS-CoV-2的趋势和新出现的新方法。逆转录酶PCR或RT-PCR是检测SARS-CoV-2疾病的金标准。Covid-19的血清阳性率采用抗体检测试验,采用ELISA和抗原检测作为快速检测。在临床实践中,疾病的初步鉴定是基于胸部x线片、计算机断层扫描和正电子发射断层扫描(PET)。随着疫情的发展,CRISPR、纳米技术解决方案和生物传感器等较新的检测方法已经成为检测SARS-CoV-2的新方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Current trends and new methods of detection of SARS-CoV-2 infection
SARS-CoV-2 is the causative agent of the global pandemic, also known as Covid-19. This virus belongs to a group of coronaviruses and has affected more than ten million people across the globe, causing nearly half a million deaths worldwide. The pandemic has spread worldwide, originating in the Wuhan Hubei province of China in 2019. The disease is a significant challenge as there is no antiviral treatment. This review will address current trends and emerging new methods for detecting SARS-CoV-2 in the laboratoryat present. Reverse transcriptase PCR or RT-PCR is the gold standard for detecting SARS-CoV-2 disease. The seroprevalence of Covid-19 is performed using antibody detection tests using ELISA and antigen detection as rapid tests. In clinical practice, preliminary disease identification is made based on Chest radiographs, computed tomography, and positron emission tomography (PET) scans. As the pandemic has progressed, newer methods of detection like CRISPR, nanotechnology-enabled solutions, and biosensors have emerged as new methods of detecting SARS-CoV-2.
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