用脉冲高电场灭活病毒

Conference Record of the 1990 IEEE Industry Applications Society Annual Meeting Pub Date : 1990-10-07 DOI:10.1109/IAS.1990.152263

A. Mizuno, T. Inoue, S. Yamaguchi, K. Sakamoto, T. Saeki, Y. Matsumoto, K. Minamiyama

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引用次数: 17

摘要

研究了脉冲强电场对液体中病毒的作用。实验采用猪水疱病病毒(SVDV)和马疱疹病毒-1 (EHV-1)。将脉冲高压反复施加到汇聚电场型电极上，该电极在平行板电极之间有针孔的绝缘板。在连续运输病毒悬浮液的针孔处形成脉冲高电场。脉冲电场(峰值30 kV/cm，指数衰减波形)作用60 ~ 120次，去离子水或牛血清中的SVDV均能有效失活。即输入电能为43.6 ~ 87.2 cal/cm/sup /。脉冲灭活后，SVDV蛋白壳的形状没有改变，但SVDV核心所含RNA消失，可见空心颗粒。当脉冲施加次数超过45次或输入电能大于32.7 cal/cm/sup /时，最小基本介质(minimum essential medium, MEM)中的EHV-1也能有效灭活。可以观察到EHV-1周围的包膜和由DNA组成的EHV-1核心的明显损伤。

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Inactivation of viruses using pulsed high electric field

The effect of pulsed high electric field on viruses in liquid was studied. Swine vesicular disease virus (SVDV) and equine herpes virus-1 (EHV-1) were used in the experiment. Pulsed high voltage was applied repetitively to the converged electric field type electrode, which had an insulating plate with pinholes between parallel-plate electrodes. A pulsed high electric field was formed at the pinhole through which the virus suspension was continuously transported. SVDV in deionized water or in bovine serum could be inactivated effectively when the pulsed electric field (crest value of 30 kV/cm, exponential-decay waveform) was applied 60 to 120 times. or the input electrical energy was 43.6 to 87.2 cal/cm/sup 3/. After inactivation by the pulse application, the shape of the protein shell of SVDV did not change, but RNA contained in the core of SVDV disappeared, and hollow particles were observed. EHV-1 in minimum essential medium (MEM) could also be inactivated effectively when the pulse application number exceeded 45, or the input electrical energy was more than 32.7 cal/cm/sup 3/. Apparent damage to the envelope around EHV-1 and to the core of EHV-1 consisting of DNA could be observed.<>

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Conference Record of the 1990 IEEE Industry Applications Society Annual Meeting

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