黄鳝抗血清的制备及性质研究。

J. Weiland, W. Shelver
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引用次数: 0

摘要

病原真菌引起的甜菜根腐病的评价方法历来依赖于目测评价。为了建立一种检测和定量耳蜗隐菌根腐病的辅助方法,我们在用耳蜗隐菌细胞壁制备物免疫的家兔体内制备了抗血清。利用酶联免疫吸附试验(ELISA)进行的特异性试验表明,抗血清对A. cochlioides和A. euteich - es都有很强的反应,但对对甜菜无致病性的卵菌、感染甜菜的丝状真菌或健康甜菜提取物的反应较弱。1:20 00稀释的血清足以在受感染的甜菜幼苗中检测出黄叶拟虫。从美国明尼苏达州的一个打桩站获得的甜菜根,表现出由黄斑拟虫引起的成虫根腐病症状,经检测该病原体的存在呈阴性。该抗血清为大田和温室栽培甜菜中黄芪的检测和根腐病的免疫化学研究提供了新的工具。关键词:甜菜根,黑根,ELISA,检测,卵菌
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Production and Characterization of Antiserum to Aphanonlyces cochlioides.
Methods for the evaluation of root rot disease in sugarbeet caused by pathogenic fungi historically have relied on visu­ al assessment. In an initial attempt to develop complemen­ tary means to detect and quantitate root rot disease caused by Aphanomyces cochlioides, antiserum was produced in rabbits that had been immunized with a cell-wall prepara­ tion of this organism. Specificity tests using enzyme-linked immunosorbant assay (ELISA) indicate that the antiserum is strongly reactive with both A. cochlioides and A. euteich­ es, but weakly with oomycetes non-pathogenic to sugar­ beet, with fIlamentous fungi that infect sugarbeet, or with extracts prepared from healthy sugarbeet. A 1:2,000 dilu­ tion of the serum was sufficient to readily detect A. cochlioides in infected sugarbeet seedlings. Sugarbeet roots obtained from a piling station in Minnesota, USA that exhibited adult root rot symptoms characteristic of those caused by A. cochlioides tested negative for the presence of this pathogen. The antiserum provides an additional tool for the detection of A. cochlioides in field and greenhouse­ grown sugarbeet and for immunochemical investigations of root rot disease. Additional Key Words: Beta vulgaris, Black-root, ELISA, detection, oomycete
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