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JURNAL NUKLEUS PETERNAKAN Pub Date : 2022-06-30 DOI:10.35508/nukleus.v9i1.5490

Biqshierz V.W Koelima, Henderiana L. L. Belli, J.N. Kihe, W. M. Nalley

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摘要

本研究的目的是研究添加细胞外冷冻保护剂的杜洛克猪在改性三卵黄中，平衡时间对冷冻精液质量的影响。每周两次用按摩的方法收集两名年龄±2.5岁的杜洛克男性的精液，他们身体健康，接受过精液收集方面的培训。采用完全随机设计，4个试验，3个处理T1:平衡时间为1小时，T2:平衡时间为2小时，T3:平衡时间为3小时，温度为50℃。观察参数为运动、活力、异常、MPU和恢复率(RR)。结果显示，平衡后2小时的活动力、活力、异常、MPU、RR(25.63±3.15%、55.86±5.41%、4.92±0.53%、56.69±5.64%、36.61±4.49%)高于1、3小时(20.00±3.54%、39.28±9.89%、7.93±0.76%、40.21±9.39%、28.57±5.05%)和(15.00±4.08%、32.19±7.0%、8.41±0.70%、32.71±7.07%、21.43±5.83%)，差异均有统计学意义(P<0.05)。综上所述，杜roc猪精子在三改性稀释液中平衡2 h后的活动力达到64.375%，解冻后达到25.63%，但未达到40%的人工授精要求。综上所述，杜洛克猪在三改性稀释液中平衡2 h后精子活力达到64.375%，解冻后达到25.63%，但未达到40%的人工授精精子活力标准。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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PENGARUH LAMA EKUILIBRASI TERHADAP KUALITAS SEMEN BEKU BABI DUROC DALAM PENGENCER TRIS-MODIFIKASI DENGAN PENAMBAHAN KRIOPROTEKTAN (Effect of equilibration time on frozen sement quality of the duroc pig in tris diluents modification With.....

The purpose of this study was to test the influence of the equilibration time on the frozen semen quality of duroc pig in modified tris-egg yolk with the addition of extracellular cryoprotectants. Semen is collected twice per week using massage methods from two duroc males aged ±2.5 years which in good health and have been trained in semen colection. The design used is a completely randomized design with 4 tests and 3 treatments T1: equilibration time of 1 hour, T2: equilibration time of 2 hours, T3: equilibration time of 3 hours at a temperature of 50C. Observed parameters were motility, viability, abnormality, MPU, and recovery rate (RR). The results showed that motility, viability, abnormalities, MPU, and RR were higher (P<0.05) at 2 hours of equilibration (25.63±3.15%, 55.86±5.41%, 4.92±0.53%, 56.69±5.64%, 36.61±4.49%) compared to 1 and 3 hours (20.00±3.54%, 39.28±9.89%, 7.93±0.76%, 40.21±9.39%, 28.57±5.05%) and (15.00±4.08%, 32.19±7.0%, 8.41±0.70%, 32.71±7.07%, 21.43±5.83%). It was concluded the motility of duroc pig spermatozoa in the tris-modified diluents after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the requirement of 40% spermatozoa motility for artificial insemination. It was concluded that the sperm motility of Duroc pig in tris-modified diluent after 2 hours of equilibration reached 64.375% and 25.63% post thawing, but did not reach the standard of 40% spermatozoa motility for artificial insemination.

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JURNAL NUKLEUS PETERNAKAN

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