THE SIGNIFICANCE OF ORAL FLUID MARKERS IN THE CELLULAR AGING EVALUATION IN GERONTOSTOMATOLOGICAL SYNDROMES

This study is devoted to the study of biomarkers of aging in oral fluid in age-associated dental diseases. 63 patients were examined, who were divided into three groups depending on the clinical syndrome: group 1 – occlusive syndrome (increased tooth abrasion, wedge-shaped defects), group 2 – inflammatory periodontal syndrome (chronic generalized periodontitis), group 3 – dystrophic syndrome (leukoplakia, candidiasis stomatitis). The age of patients in these groups was 60–74 years, which corresponds to an elderly age. The control group included 20 healthy volunteers under the age of 40. The content of tumor necrosis factor (TNF), interleukin-6 (IL-6), and protein p53 were determined in the oral fluid. The concentration of these proteins was determined by multiparametric fluorescence analysis with magnetic microspheres (xMAP technology, Luminex 200, USA) using the ProcartaPlex Apoptosis 6-Plex Human ProcartaPlex ™ Panel and ProcartaPlex test systems Human Cytokine/Chemokine (Invitrogen, USA). According to a number of authors, these parameters indirectly characterize the processes of cellular aging. We have identified laboratory markers of oral fluid characteristic of age-associated syndromes, for occlusive syndrome it is salivary TNF, for inflammatory periodontal – TNF and possibly IL-6, for dystrophic syndrome – protein p53. In addition, a correlation was found between age and the severity of the process. These salivary markers can be further considered as candidates for inclusion in laboratory monitoring when evaluating the effectiveness of new technologies for personalized dental treatment of patients of older age groups.