胞囊藻SP.突变体H114Q的天线蛋白CP47持续谱孔燃烧

T. Polívka, D. Engst, J. Dian, P. Kroh, J. Pšenčík, M. Vácha, L. Nedbal, Werner Vermaas, J. Hála
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引用次数: 0

摘要

光谱空穴燃烧是研究光合系统中快速弛豫过程(如激发态能量传递、电子传递等)的有力工具。采用瞬态烧孔法(THB)对紫色细菌和绿色植物的反应中心吸收光谱进行了系统的研究。PSII粒子的THB荧光用[2]表示。持续光谱空穴燃烧(PSHB)可以测定空穴燃烧机理、EET速率常数、电子-声子耦合和蛋白质声子频率。在PSII[3]的天线配合物CP43和CP47、紫色光合细菌[4]的B800-850和绿硫光合细菌[5]的叶绿体中测量了荧光PSHB。最近在[6]中报道了PSII的CP43荧光光谱中激光诱导的空穴填充。这些数据是用野生型生物获得的。本研究利用Synechocystis sp. PCC 6803 CP47复合体的H114Q突变,利用荧光PSHB对光合天线中的EET进行了研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Persistent Spectral Hole Burning In The Antenna Protein CP47 Of Synechocystis SP. Mutant H114Q
Spectral hole-burning is powerful tool for the study of fast relaxation processes (e.g. excited energy transfer - EET, electron transport - e.t.) in photosynthetic systems. Fast e.t. was systematically studied by transient hole-burning (THB) in absorption spectra of reaction centra in purple bacteria and green plants [1]. The THB in fluorescence of PSII particles was described in [2]. Persistent spectral hole-burning (PSHB) enabled to determine the hole-burning mechanism, the EET rate constants, electron-phonon coupling and frequency of protein phonons. The PSHB in fluorescence has been measured in antenna complexes: CP43 and CP47 of PSII [3], B800-850 of purple photosynthetic bacteria [4] and in chlorosomes of green sulphur photosynthetic bacteria [5]. Laser induced hole filling in fluorescence spectra of CP43 of PSII was presented recently in [6]. These data were obtained using wild type organisms. Here, we report an investigation of EET by fluorescence PSHB in photosynthetic antenna using H114Q mutation in the CP47 complex of Synechocystis sp. PCC 6803.
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