高、低压脉冲组合基因电转移诱导组织焦耳加热的建模

I. Lacković
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引用次数: 0

摘要

基因电转移是一种安全有效的基因治疗技术。它依靠局部传递的电脉冲将先前注射的外来DNA转移到细胞中。施加脉冲在组织中产生电场(电穿孔),并由于焦耳效应而产生热量。在我们的研究中,我们使用有限元方法计算了典型的电传递脉冲方案(即100微秒高压脉冲和400毫秒低压脉冲的组合,脉冲滞后时间为1秒)在各向同性组织(如肝脏)和各向异性组织(如骨骼肌)中的电场和温度分布。对于平行板电极,结果表明,由于高压脉冲,组织体的温度升高可以忽略不计,而由于低压400毫秒长的脉冲,温度升高可达几°C,这取决于组织的导电性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modeling of Joule heating in tissues induced by high- and low-voltage pulse combinations for gene electrotransfer
Gene electrotransfer is a promising technique for safe and efficient gene therapy. It relies on locally delivered electric pulses to transfer previously injected foreign DNA into the cell. Applied pulses induce electric field in tissue (electroporation) and concomitant heat generation due to Joule effect. In our study we used finite element method to calculate electric field and temperature distribution during typical electrotransfer pulsing protocol (i.e. combination of 100 microsecond high-voltage pulse and 400 millisecond low-voltage pulse with pulse lag time of 1 second) in isotropic tissue (e.g. liver) and anisotropic tissue (e.g. skeletal muscle). For parallel plate electrodes results show negligible temperature rise in tissue bulk due to high voltage pulse and up to a few °C rise due to the low-voltage 400 ms-long pulse, depending on tissue conductivity.
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