检测假蜂蜜产品的简易快速筛选试验

N. Johari, Nur Suhanawati Ashaari, Mohd Razif Mamat, A. Muhamad
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引用次数: 1

摘要

尽管食品分析平台和CODEX标准不断进步,但蜂蜜欺诈仍然是一个全球性问题。即使有严格的指导方针,在地面上控制进入的蜂蜜产品被证明是具有挑战性和难以解决的。虽然大多数分析平台足以检测假冒蜂蜜产品,但也需要开发一种快速筛选测试来支持现场监管活动。根据生物化学原理研制的一种化学试剂已被证明能够区分合成蜂蜜和原始蜂蜜。该反应只需要三滴蜂蜜产物与少量的试剂混合物反应,在2分钟的反应时间内,蛋白质和其他分子发生了颜色变化和沉淀。对包括几种无刺蜜蜂和Apis物种在内的原料蜂蜜样品进行的测试结果表明,与白糖、黄金糖浆、醋、酸橙汁和大蕉混合制成的假蜂蜜相比,蜂蜜产生了沉淀,这导致了明显的有机相,表明缺少天然蜂蜜的生物物质。在使用复杂的分析平台进行鉴定之前,原料蜂蜜和假蜂蜜与试剂反应的差异可以用于实时的现场筛选活动。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Simple and Rapid Screening Test to Detect Fake Honey Product
: Despite advancement in food analytical platforms and availability of CODEX standard, the fraud honey relentlessly remains as global issue. Even with stringent guidelines, controlling the incoming honey product at ground level proved to be challenging and difficult to tackle. While most analytical platforms are powerful enough to detect counterfeit honey products, there is also a need to develop a rapid screening test to support field regulatory activity. A chemical reagent developed based on biochemistry principle has proved to be able to differentiate synthetic honey product from its raw counterpart. The reaction required only three drops of honey product to react with few drops of reagent mixtures which resulted in a change of color and precipitation of proteins and other molecules within 2 min reaction. Test performed on raw honey samples inclusive of several stingless bees and Apis species honey resulted in a precipitation as compared to fake honey generated from a blend of white sugar, golden syrup, vinegar, lime juice and plantain which resulted in clear organic phase indicating missing natural honey biological matters. The difference between raw honey and fake honey reaction with the reagents can be utilized for real time on field screening activity before proceeding with authentication using complex analytical platforms.
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