体外酸性介质电穿孔后细胞膜通透性和细胞存活率的研究

2018 EMF-Med 1st World Conference on Biomedical Applications of Electromagnetic Fields (EMF-Med) Pub Date : 2018-09-01 DOI:10.23919/EMF-MED.2018.8526061

Tjaša Potočnik, D. Miklavčič, A. M. Lebar

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引用次数: 1

摘要

电穿孔是一种在细胞上施加足够振幅和持续时间的电场使细胞膜瞬间通透的方法。由于电穿孔在体内应用的微环境可能是酸性的，我们研究了酸性电穿孔和回收介质的影响。我们观察到，在生长(pH 7.8)或酸性(pH 6.5)培养基中，电穿孔并允许恢复的细胞的渗透阈值没有差异。相比之下，暴露于脉冲振幅大于渗透阈值的细胞的存活率有统计学意义的差异。在相同电压下，与在生长介质中电穿孔和恢复后的存活率相比，使用酸性电穿孔和恢复介质的细胞存活率更高。我们的结果表明，膜修复的有效性取决于细胞外pH值的不同。

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Cell membrane permeabilization and cell survival after electroporation in acidic media in vitro

Electroporation is a method in which electric field of sufficient amplitude and duration applied to cells causes transient permeabilization of the cell membrane. Due to possibility, that microenvironment in applications of electroporation in vivo is acid, we studied effects of acid electroporation and recovery media. We observed no difference in the permeabilization threshold of cells which were electroporated and allowed to recover in growth (pH 7.8) or acid (pH 6.5) media. In contrast, statistically significant difference was observed in survival of cells that were exposed to pulse amplitudes greater than permeabilization threshold. At equal voltages applied survival of cells was greater if acid electroporation and recovery media were used compared to survival after electroporation and recovery in growth media. Our results imply that there is difference in membrane repair effectiveness depending on extracellular pH.

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来源期刊

2018 EMF-Med 1st World Conference on Biomedical Applications of Electromagnetic Fields (EMF-Med)

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