Differentiation of phytoplankton populations by in vivo fluorescence

In order to develop an in situ algae fluorescence auto-analyzer,the discriminating technology for phytoplankton populations was established by in vivo chlorophyll fluorescence excitation spectra composed of 12 excitation wavelengths(400,430,450,460,470,480,490,510,525,550,570,and 590 nm) of the algae occurring frequently in the coastal waters of China and a multivariate linear regression model.The linear regression model was solved by non-negative least squares.Some samples were tested.For 79 single division algal samples,96% samples were accurately discriminated,with recovery efficiency above 68%,and the recovery efficiency of 85% samples was above 80%.For 17 mixed division algal samples,76% samples were accurately determinated,with recovery efficiency above 74%.