DEVELOPMENT AND VALIDATION OF STABILITY INDICATING UPLC METHOD FOR SIMULTANEOUS QUANTIFICATION OF IMIDACLOPRID, THIRAM AND CARBOXIN IN PESTICIDE FORMULATION

A novel stability-indicating ultra-performance liq uid chromatography (UPLC) method has been developed and validated for quantification of Imidacloprid, Thiram and carboxin in pesticide formulation (FS), using Poroshell 120 EC-C18 (100 m m × 4.6 mm, 2.7μm) column. Mixture of Water: Methanol (40:60 v/v) was used as mobile phase. The flow rate was kept 0.40 ml/min and detection was carried out at 250 nm. The limit of detection was 0 .00 6mg/ml, 0.0007mg/ml and 0.0007mg/ml for Imidacloprid, Thiram and Carboxin respectively. The limit of quantitation values was 0.0019mg/ml, 0.0015mg/ml and 0.0015mg/ml for Imidacloprid, Thira m and Carboxin respectively. The linearity of proposed method was investigated in the range of 0. 0019-0.596mg/ml (r =0.9997), 0.0015-0.178mg/ml (r=0.9997) and 0.0015-0.175mg/ml (r =0.9997) for Imidacloprid, Thiram and Carboxin resp ctively. The percentage recovery found to be in range from 9 8.19.9 %, 98.3-100.7% and 98.6-99.6% for Imidacloprid, Thiram and Carboxin respectively. The % RSD values for intraday precision study and interday precision study were <1.66, <2.0 and <2.0 for Imidacloprid, Thiram and Carboxin respectively, as per modified Horwitz equation as r equirements by CIPAC. The method was found to be specific, linear, precise, accurate and robust. This method is also useful for quantification of Imidacloprid, Thiram and Carboxin in their single o r combination formulated products, with different strengths and different formulation types.