Masayuki Horayama, Tomoki Ohkubo, K. Arai, K. Kabayama, T. Fujii, H. Kimura
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Pinpoint chemical stimulation at a single-cell scale by microfluidic techology
In this study, we have developed a microfluidic device to control chemical stimulation to a cell population at a single cell scale for cell-based assay. The device consists of a cell culture chamber and microfluidic probe (MFP) channels which are possible to control pinpoint chemical stimulation in the microspace. The device has been evaluated by a computer simulation using Finite Element Method (FEM) and a flow control experiment. A control of chemical stimulation from single cell scale to a cell population scale in the chamber can be possible by regulating flow ratio and channel size of the microfluidic probe.
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