Extraction and Analysis of Nicotine in the Urine of Active Smokers after given by Vitamin C

: Nicotine is a class of addictive compounds that can cause dependence. Vitamin C contains antioxidants that can ward off free radicals in the body. Nicotine extraction and analysis are a series of processes that are indispensable in order to produce nicotine in optimal concentrations. This study aims to extract and analyze nicotine in the urine of active smokers after giving vitamin C. The descriptive method was developed for the extraction of nicotine in urine. Preliminary test using Cyanogen bromide showed the presence of nicotine in the urine of smokers for 7 days. The same thing is evidenced by the thin layer chromatography data resulting in an average Rf value of 0.6. The concentration of nicotine in urine decreased from the first day of vitamin C administration to day 7. This indicates that there is an effect of vitamin C on the urine of active smokers.