Efficacy of Nerium oleander Leaves extract on controlling Meloidogyne incognita in-Vitro and in-Vivo

Experiments were carried out to evaluate the nematicidal effect of Oleander (Nerium oleander) extracts on controlling root-knot nematode Meloidogyne incognita in-Vitro and in-Vivo . Five different organic solvents i.e., Petroleum Ether, Chloroform, Acetone, Ethyl Alcohol and Methyl Alcohol were used. Plant extracts screened for nematicidal activity against egg hatching and mortality of second-stage juveniles of M. incognita under laboratory conditions and on nematode infecting tomato plants under greenhouse conditions. Results showed significantly effect of all tested oleander extracts on egg hatching and larvae mortality under laboratory conditions. A great reduction in number of galls; eggmasses; females/root system of tomato plants and number of second stage juveniles (J2S)/250 g soil was recorded compared to the treated plants with nematode alone. Tomato plant growth parameters and oxidative enzymes were markedly enhanced compared to the non-treated control.