{"title":"(L-Ala-D-Glu-L-Lys-D-Ala-Gly)n的免疫化学性质兔抗体具有有限异质性。","authors":"A R Zeiger, P H Maurer","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A rabbit antiserum of restricted specificity elicited by the sequential polypeptide, (L-Ala-D-Glu-L-Lys-D-Ala-Gly)n, was purified by column chromatography using Sepharose 4B to which the inhibitor, Gly-L-Ala-D-Glu-Gly, was covalently attached. Approximately 95% of the antibody activity was retained on the immunoabsorbant. After elution, the purified antibody was shown by electrofocusing in a sucrose gradient to be of restricted heterogeneity. The entire antibody fraction was contained within the pH range of 6.9-8.9. The normal immunoglobulin pool from this rabbit, on the other hand, was found by electrofocusing to be distributed throughout the pH range studied (3.6-9.2).</p>","PeriodicalId":23768,"journal":{"name":"Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie","volume":"149 2-4","pages":"151-6"},"PeriodicalIF":0.0000,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The immunochemistry of (L-Ala-D-Glu-L-Lys-D-Ala-Gly)n: II. Rabbit antibodies of restricted heterogeneity.\",\"authors\":\"A R Zeiger, P H Maurer\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A rabbit antiserum of restricted specificity elicited by the sequential polypeptide, (L-Ala-D-Glu-L-Lys-D-Ala-Gly)n, was purified by column chromatography using Sepharose 4B to which the inhibitor, Gly-L-Ala-D-Glu-Gly, was covalently attached. Approximately 95% of the antibody activity was retained on the immunoabsorbant. After elution, the purified antibody was shown by electrofocusing in a sucrose gradient to be of restricted heterogeneity. The entire antibody fraction was contained within the pH range of 6.9-8.9. The normal immunoglobulin pool from this rabbit, on the other hand, was found by electrofocusing to be distributed throughout the pH range studied (3.6-9.2).</p>\",\"PeriodicalId\":23768,\"journal\":{\"name\":\"Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie\",\"volume\":\"149 2-4\",\"pages\":\"151-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1975-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
由序列多肽(L-Ala-D-Glu-L-Lys-D-Ala-Gly)n诱导的兔抗血清，用与抑制剂gly - l - ala - d - glly共价结合的Sepharose 4B柱层析纯化。大约95%的抗体活性保留在免疫吸收剂上。洗脱后，经糖梯度电聚焦表明纯化抗体具有有限的异质性。整个抗体部分的pH值在6.9-8.9之间。另一方面，通过电聚焦发现这只兔子的正常免疫球蛋白池分布在所研究的pH范围内(3.6-9.2)。
The immunochemistry of (L-Ala-D-Glu-L-Lys-D-Ala-Gly)n: II. Rabbit antibodies of restricted heterogeneity.
A rabbit antiserum of restricted specificity elicited by the sequential polypeptide, (L-Ala-D-Glu-L-Lys-D-Ala-Gly)n, was purified by column chromatography using Sepharose 4B to which the inhibitor, Gly-L-Ala-D-Glu-Gly, was covalently attached. Approximately 95% of the antibody activity was retained on the immunoabsorbant. After elution, the purified antibody was shown by electrofocusing in a sucrose gradient to be of restricted heterogeneity. The entire antibody fraction was contained within the pH range of 6.9-8.9. The normal immunoglobulin pool from this rabbit, on the other hand, was found by electrofocusing to be distributed throughout the pH range studied (3.6-9.2).
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
