便携式荧光仪(PorFloR™)的开发与测试

Vivek B. Borse, A. S. Patil, R. Srivastava
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引用次数: 8

摘要

PorFloR™是一款即时便携式荧光检测仪。它是一种小型手持设备,记录荧光斑的强度和荧光斑在二维固体基材(如纸条)上的荧光强度变化。目前,许多基于荧光的侧流免疫测定(LFIA)技术被用于检测各种疾病生物标志物。大多数技术依赖于可见比色检测过程。从这种技术得到的结果是定性的或半定量的。因此,我们开发了便携式,易于使用,低成本,即时的条带读取器,可以定量精确地检测表面的外观以及荧光强度的变化。从设备的输出可以编程,以显示存在以及分析物的浓度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development and testing of portable fluorescence reader (PorFloR™)
PorFloR™ is a point-of-care portable fluorescence reader. It is a small handheld device that records the intensity of fluorescence spot and change in fluorescence intensity of the spot on the solid 2D base (e.g. paper strips) material. Nowadays, numerous fluorescence based lateral flow immunoassay (LFIA) techniques are utilized for the detection of various disease biomarkers. Most of the techniques depend on visible colorimetric detection process. Results obtained from such techniques are qualitative or semi-quantitative. Thus, we have developed the portable, easy-to-use, low cost, point-of-care strip reader that can detect the appearance as well as change in fluorescence intensity on the surface with quantitative precision. The output from device can be programmed to display the presence as well as concentration of an analyte.
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