Characterization of Cell Microcapsules and Preparation via Digital Coaxial Injection

Cell microcapsules are prepared and characterized by digital injection of coaxial micro-fluidics to better control cells number and reduce the redundancy space in microcapsules. The results show that the diameter of the inner and outer nozzles of the micro coaxial nozzle have a significant effect on the particle size of the cell microcapsules, particle size distribution and the number of encapsulated cells. The particle size of the lymphocyte microcapsules increases linearly with the diameter of the outer nozzle, not significantly influenced by the change of the diameter of the inner nozzle. The number of lymphocytes in the microcapsules increases exponentially with the inner nozzle diameter, and is not significantly affected by the diameter of the outer nozzle. So, we can flexibly control the particle size of the microcapsules and the number of encapsulated cell, reduce the redundant space by the ratio between the inner and outer nozzle diameters.