实验性转移性前列腺癌盆腔淋巴结的形态学参数

V. Astashov, N. Kulchenko
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引用次数: 0

摘要

研究目的。实验性转移性前列腺癌盆腔淋巴结结构和功能改变的鉴定。材料和方法。该研究包括30只3个月大的雄性小鼠。所有动物(n = 30)分为2组。第一组(主要的一组,n = 20)包括通过将埃利希肿瘤移植到前列腺实质中产生转移性前列腺癌模型的小鼠。这些动物进一步分为两个相等的亚组:1a (n = 10) -小鼠在第7天退出实验;第18天,1b (n = 10)只小鼠退出实验。第二组(n = 10)包括未被操纵的小鼠,它们构成对照组。实验结束后,光镜下对两组小鼠前列腺及局部(盆腔)淋巴结进行形态学分析。使用Statistica 8.0软件包对结果进行统计处理。p < 0.05认为差异有统计学意义。与对照组(1a组和1b组)相比,前列腺实质几乎完全被非典型细胞所取代,这表明前列腺内存在肿瘤进程。1a组大鼠盆腔淋巴结数是2组的1.9倍,1b组大鼠盆腔淋巴结数是2组的2.7倍(p < 0.01)。第一组动物盆腔淋巴结肥大细胞比例较对照组增加了121% (p < 0.01)。同时,我们揭示了肥大细胞与髓窦肿瘤细胞和免疫母细胞数量的直接关系,这表明组织嗜碱性粒细胞参与了肿瘤转移。我们还记录到,与对照组相比,1a组动物的区域淋巴结髓窦面积增加了12.4% (p < 0.05), 1b组动物的区域淋巴结髓窦面积增加了20.2% (p < 0.01),该区域肥大细胞数量增加,这表明肥大细胞可能参与了淋巴管生成。肥大细胞可能在前列腺恶性肿瘤的发展中发挥潜在的作用。转移性前列腺癌动物盆腔淋巴结肥大细胞数量增加。肥大细胞数量的增加和肿瘤细胞数量的增加与免疫母细胞的直接依赖表明,组织嗜碱性粒细胞参与了转移的扩散。可以假设肥大细胞浸润淋巴结可能与前列腺癌预后不良有关,因为这些粒细胞有助于肿瘤转移。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Morphometric parameters of pelvic lymph nodes in experimental metastatic prostate cancer
The study purpose. Identification of structural and functional changes in pelvic lymph nodes in experimental metastatic prostate cancer.Materials and methods. The study included 30 male mice aged 3 months. All animals (n = 30) were divided into 2 groups. The first group (the main one, n = 20) included mice that had a model of metastatic prostate cancer created by transplanting an Ehrlich tumor into the prostate parenchyma. These animals were further divided into two equal subgroups: 1a (n = 10) – mice were withdrawn from the experiment on day 7; 1b (n = 10) – mice were withdrawn from the experiment on day 18. The second group (n = 10) included mice that were not manipulated, they made up the control group. After the end of the experiment, morphological analysis of the prostate gland and regional (pelvic) lymph nodes in mice of both groups was performed using light microscopy. Statistical processing of the results was carried out using the Statistica 8.0 software package. The differences were considered statistically significant at p < 0.05.Results. When compared to the control group the mice of groups 1a and 1b, the prostate parenchyma was almost completely replaced by atypical cells, which demonstrates the presence of neoplastic processes in the prostate gland. In group 1a mice, the number of pelvic lymph nodes was 1.9 times as large, and in group 1b animals, this indicator was 2.7 times higher compared to the second group (p < 0.01). In animals of the first group, the proportion of mast cells in the pelvic lymph nodes increased by 121 % compared to the control group (p < 0.01). At the same time, we revealed a direct relationship of mast cells with the number of tumor cells and immunoblasts in the medullar sinuses, which indicates the participation of tissue basophils in tumor metastasis. We also recorded a significant increase in the area of the medullar sinuses of regional lymph nodes in animals of groups 1a by 12.4 % (p < 0.05) and 1b by 20.2 % (p < 0.01) against the background of an increase in the number of mast cells in this zone compared to the control group, which indicates the possible participation of mast cells in lymphangiogenesis.Conclusion. Mast cells can potentially play a role in the development of malignant neoplasms of the prostate gland. The number of mast cells increases in pelvic lymph nodes in animals with a created model of metastatic prostate cancer. The obtained direct dependence of the increase in the number of mast cells and the increase in the number of tumor cells with immunoblasts suggests the participation of tissue basophils in the spread of metastases. It can be assumed that infiltration of lymph nodes by mast cells may correlate with a poor prognosis of prostate cancer, since these granulocytes contribute to tumor metastasis.
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