罗望子种子生理品质评价的四氮唑试验

Jenry Rafael Hernández, Camilo José Iguaran-Diaz, Hérmes Araméndiz-Tatis, C. Cardona-Ayala, M. Espitia-Camacho
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摘要

罗望子(Tamarindus indica)是一种原产于非洲的果树,在包括哥伦比亚在内的50多个国家种植,主要在哥伦比亚加勒比省种植,其收成为农村社区带来了可观的收入。由于有性种子存在生理休眠和发芽率差的问题,使其建立困难。本研究的目的是利用四氮唑试验来评价罗望子种子的活力,以减少测定罗望子生理品质所需的时间。从庭院树上采集成熟健康的果实,提取种子,用1%次氯酸钠消毒,使用前用大量水清洗。随后,除微孔区域外,用100号砂纸对其边缘进行打磨。完全随机设计采用3 × 3双因子排列,重复4次。分别在0.50、0.75和1.00%的浓度下测定第一个因素四氮唑浓度(%),在2、4和6小时时测定第二个因素四氮唑浓度(%)。四氮唑浓度差异无显著性,浸泡时间差异有显著性(p≤0.01),四氮唑-时间交互作用差异有显著性(p≤0.05)。因此,与传统的发芽测试相比,0.50%四氮唑浸泡6小时是测定罗望子种子生理质量的可靠选择,因为它的成本低,执行时间短。同样,用砂纸切割种子的发芽率和发芽速度指数也高于常规方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tetrazolium test to estimate the physiological quality of tamarind (Tamarindus indica L.) seeds
Tamarind (Tamarindus indica) is a fruit tree of African origin cultivated in more than 50 countries, including Colombia, mainly in the Colombian Caribbean departments, where its harvest generates significant income in rural communities. Its establishment presents a difficulty because the sexual seed presents physical dormancy and poor germination. The objective of this study was to evaluate Tamarind seed viability by using the tetrazolium test and to reduce the time needed for the determination of its physiological quality. Ripe and healthy fruits were collected from patio trees, from which their seeds were extracted, disinfected with 1% sodium hypochlorite, and washed with plenty of water before use. Subsequently, they were scarified with No. 100 sandpaper by the edges, except in the area of the micropyle. The completely random design was used in a 3x3 bifactorial arrangement, with four repetitions. The first factor, tetrazolium concentration (%), was tested at levels of 0.50, 0.75 and 1.00%, and the second factor, immersion time, at 2, 4, and 6 hours. The analysis of variance did not show significance for the tetrazolium concentration, while it did for the immersion time (p≤0.01), and the tetrazolium-time interaction (p≤0.05). Therefore, the concentration of 0.50% tetrazolium for six hours of immersion is a reliable alternative to determine the physiological quality of tamarind seeds compared to conventional germination testing due to its low cost and execution time. Likewise, the percentages of germination and germination speed index were higher when the seed was scarified with sandpaper than the conventional method.
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