壳聚糖和金纳米颗粒标记孔雀石绿免疫传感器

2020 3rd International Conference on Electron Device and Mechanical Engineering (ICEDME) Pub Date : 2020-05-01 DOI:10.1109/ICEDME50972.2020.00119

Min-Fu Wu, Min-hua Wu, Sha Li, X. Li

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引用次数: 0

摘要

研制了一种用于水和鱼样品中孔雀石绿检测的免疫传感器。在金电极(GE)表面制备了具有良好生物相容性和导电性的GNPs。将多克隆抗体固定在电极上。在最佳条件下，该免疫传感器对孔雀石绿的检测灵敏度在0.032 ~100 ng/mL范围内，缓冲液的检测限为10.7 /mL。添加水和鱼样品中孔雀石绿的回收率为84.2% ~ 113.8%，表明该免疫传感器可用于真实样品的检测。

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Labeled free Malachite Green immunosensor based on chitosan and gold nano particles

An immunosensor for the detection of malachite green in water and fish samples was developed. GNPs with good biocompatibility and good electroconductivity were fabricated onto the surface of a gold electrode (GE). The polyclonal antibody was immobilized on the electrode. Under optimal conditions, the proposed immunosensor exhibited a sensitive response to malachite green in the range of 0.032 ~100 ng/mL, with a detection limit of 10.7 /mL in buffer. Recoveries of malachite green from spiked water and fish samples ranged from 84.2% to 113.8%, indicating that the proposed immunosensor potential can be used for the detection of real samples.

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2020 3rd International Conference on Electron Device and Mechanical Engineering (ICEDME)

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