绿芦笋采后变质过程中酶活性的变化

P. Bhowmik, S. Basu, A. Alam
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引用次数: 1

摘要

新鲜芦笋(asparagus officinalis L.)在收获后迅速变质。芦笋采后早期酶促变化是导致采后变质的重要因素。我们将收获后的芦笋在25°C下保存5天,并检测了笋茎顶部和底部酸转化酶(AI)、蔗糖合成酶(SS)、谷氨酰胺合成酶(GS)和苯丙氨酸氨裂解酶(PAL)活性的变化。可溶性AI活性在贮藏第1天呈上升趋势,贮藏第5天呈下降趋势。底部蔗糖合酶活性高于顶部;它的活动连续五天每天都在下降。蔗糖含量与蔗糖合酶和蔗糖转化酶呈负相关。顶部和底部GS酶活性在整个试验期间均下降,这可能与氨积累增加有关。PAL活性的增加持续到第3天,此后下降。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Changes in Enzyme Activities During Postharvest Deterioration of GreenAsparagus Spears
Fresh asparagus (Asparagus officinalis L.) deteriorates rapidly after harvest. Early enzymatic changes that fol- low harvesting of asparagus are important factors contributing to postharvest deterioration. We held asparagus spears at 25°C for up to 5 days after harvest and examined changes in the activities of Acid invertase (AI), Sucrose Synthase (SS), Glutamine Synthetase (GS) and Phenylalanine ammonia Lyase (PAL) in both top and bottom portions of the spears. Solu- ble AI activity increased during the first day of storage and after that it decreased gradually up to five days in both top and bottom portions. Sucrose synthase activity was higher in the bottom portion than in the top; its activity declined daily for five days. Sucrose content was negatively correlated with invertase and sucrose synthase in both portions.GS enzyme ac- tivity in both top and bottom portion declined throughout the experimental period which may be related to increased am- monia accumulation. The increase in PAL activity continued until day 3 and declined thereafter.
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