Nataly Delgado-Bastidas, L. K. Lagos-Santander, Tulio César Lagos-Burbano
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引用次数: 1
摘要
枸杞,又称醋栗,因其营养价值和经济价值而广为人知。然而,由于其孤儿物种的地位,人们对其分子水平上的遗传多样性知之甚少。因此,对紫杉树种质资源遗传多样性的了解将有助于确定育种者在选择过程中可用的遗传变异水平。本研究利用6个SRR(简单序列重复)分子标记对40个角醋栗基因型的遗传变异进行了评估,这些标记是根据其在秘鲁红醋栗中较高的多态性而选择的。将收集的样品分为3个群体:DH(双单倍体系)、FT(耐尖孢镰刀菌基因型)和UDENAR (universsidad de Nariño)。使用GenAlex v. 6.5和Arlequin 3.5.2软件检测到7.33个等位基因。在所使用的6个标记中,SSR15和SSR138的信息量最大。总的来说,这些标记表明22.2%的位点是多态性的,平均期望杂合度为0.09,这被认为是低的。AMOVA分析表明,基因型内方差占总方差的100%,表明种群结构不存在。总的来说,我们得出结论,基因型之间的变异水平很低。
Genetic diversity of 40 genotypes of cape gooseberry Physalis peruviana L. using microsatellite markers
Physalis peruviana L., also called cape gooseberry, is widely known for its nutraceutical and economic importance. However, little is known about the genetic diversity of this species at the molecular level, mainly due to its status as an orphan species. Therefore, knowledge of the genetic diversity of germplasm collections of P. peruviana will allow determining the level of genetic variability that is available to breeders for selection processes. This study assessed the genetic variation present in 40 cape gooseberry genotypes using six SRR (simple sequence repeats) molecular markers selected based on their high polymorphism in P. peruviana L. The collection was divided into three populations: DH (double haploid lines), FT (Fusarium oxysporum-tolerant genotype), and UDENAR (Universidad de Nariño). We detected 7.33 alleles using GenAlex v. 6.5 and Arlequin 3.5.2 software. Among the six markers used, SSR15 and SSR138 were the most informative. Together, these markers indicated that 22.2% of loci were polymorphic with an average expected heterozygosity of 0.09, which is considered low. The AMOVA showed that the variance within genotypes contributes to 100% of the total variance, indicating the absence of population structure. Overall, we conclude that the level of variability among genotypes is low.