{"title":"增强检测细胞路径在时空图的非侵入性显微镜的人视网膜","authors":"Johnny Tam, A. Roorda","doi":"10.1109/ISBI.2010.5490109","DOIUrl":null,"url":null,"abstract":"Spatiotemporal (ST) plots have been used for studying cell motion in the microcirculation. However, ST plots are typically applied to invasive imaging methods. Noninvasive video microscopy of the human retinal capillaries can be performed using an Adaptive Optics Scanning Laser Ophthalmoscope, but direct implementation of ST plots is difficult due to low contrast and high noise. We introduce motion contrast enhancement to enhance detection of cell paths, and enable ST plot analysis. Using features generated by the motion contrast enhancement, a method for automatic extraction of cell paths was developed. Our results show that motion contrast is an important precursor step to ST plot analysis for videos with low signal to noise ratios.","PeriodicalId":250523,"journal":{"name":"2010 IEEE International Symposium on Biomedical Imaging: From Nano to Macro","volume":"10 12","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2010-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"Enhanced detection of cell paths in spatiotemporal plots for noninvasive microscopy of the human retina\",\"authors\":\"Johnny Tam, A. Roorda\",\"doi\":\"10.1109/ISBI.2010.5490109\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Spatiotemporal (ST) plots have been used for studying cell motion in the microcirculation. However, ST plots are typically applied to invasive imaging methods. Noninvasive video microscopy of the human retinal capillaries can be performed using an Adaptive Optics Scanning Laser Ophthalmoscope, but direct implementation of ST plots is difficult due to low contrast and high noise. We introduce motion contrast enhancement to enhance detection of cell paths, and enable ST plot analysis. Using features generated by the motion contrast enhancement, a method for automatic extraction of cell paths was developed. Our results show that motion contrast is an important precursor step to ST plot analysis for videos with low signal to noise ratios.\",\"PeriodicalId\":250523,\"journal\":{\"name\":\"2010 IEEE International Symposium on Biomedical Imaging: From Nano to Macro\",\"volume\":\"10 12\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-04-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2010 IEEE International Symposium on Biomedical Imaging: From Nano to Macro\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/ISBI.2010.5490109\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2010 IEEE International Symposium on Biomedical Imaging: From Nano to Macro","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ISBI.2010.5490109","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Enhanced detection of cell paths in spatiotemporal plots for noninvasive microscopy of the human retina
Spatiotemporal (ST) plots have been used for studying cell motion in the microcirculation. However, ST plots are typically applied to invasive imaging methods. Noninvasive video microscopy of the human retinal capillaries can be performed using an Adaptive Optics Scanning Laser Ophthalmoscope, but direct implementation of ST plots is difficult due to low contrast and high noise. We introduce motion contrast enhancement to enhance detection of cell paths, and enable ST plot analysis. Using features generated by the motion contrast enhancement, a method for automatic extraction of cell paths was developed. Our results show that motion contrast is an important precursor step to ST plot analysis for videos with low signal to noise ratios.