[大肠杆菌中乙肝病毒基因组的克隆]。

Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles Pub Date : 1978-12-01

A Fritsch, C Pourcel, P Charnay, P Tiollais

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引用次数: 0

摘要

将乙型肝炎病毒(Dane颗粒)全基因组体外插入噬菌体lambda gtWES的基因组中。重组DNA分子在大肠杆菌中克隆成功。杂交噬菌体的扩增使制备大量乙型肝炎病毒DNA成为可能。讨论了利用该重组噬菌体的可能性。

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[Cloning of the hepatitis B virus genome in Escherichia coli].

The whole genome of the hepatitis B virus (Dane particles) was inserted in vitro in the genome of the bacteriophage lambda gtWES . LAMBDA B. The recombinant DNA molecule was cloned in E. coli. Amplification of the hybrid bacteriophage enables the preparation of large amounts of hepatitis B virus DNA. The possibilities offered by the utilization of this recombinant bacteriophage are discussed.

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Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles

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