三七皂苷和丹参酮IIA对炎症相关性结直肠癌小鼠的保护作用及对COX-2表达的抑制作用

Q3 Medicine

Digital Chinese Medicine Pub Date : 2021-03-01 DOI:10.1016/j.dcmed.2021.03.007

Cao Wen , Zhou Xiaoqing

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引用次数: 3

摘要

目的探讨三七皂苷(NGS)和丹参酮IIA (TSN)对小鼠炎症相关性结直肠癌(IRCC)的预防作用及其可能的作用机制。方法雄性C57BL/6小鼠88只，随机分为11组，每组8只。建立偶氮甲烷氧化物+葡聚糖硫酸盐(AOM + DSS)模型对照(model)、NGS低剂量(l-NGS)、NGS中剂量(m-NGS)、NGS高剂量(h-NGS)、TSN低剂量(l-TSN)、TSN中剂量(m-TSN)、(NGS+TSN)中剂量[m-(NGS +TSN)]、(NGS+TSN)高剂量[h-(NGS +TSN)]和空白组。前10组腹腔注射AOM诱导炎性结肠癌，空白组腹腔注射0.9% NaCl溶液。前10组从第5天开始连续饮用2.5% DSS钠水溶液，共3个周期(1个周期:5天，每3周)，空白组自由饮水。给药组分别给予NGS(低、中、高剂量)、TSN(低、中、高剂量)或NGS + TSN(低、中、高剂量)，模型组和空白组灌胃生理盐水至实验结束。结果(1)h-NGS、m-TSN、h-TSN、m-(NGS + TSN)、h-(NGS + TSN)、h-(NGS + TSN)组IRCC小鼠的存活率显著高于其他各组(P <0.05)。(2) h-(NGS + TSN)、m-TSN、l-NGS组肿瘤发生率均显著低于模型组(P <0.05)。(3) m-(NGS + TSN)和h-(NGS + TSN)组小鼠肿瘤组织中COX-2的表达水平均显著低于模型组(P <0.05)。结论m-TSN和h-(NGS + TSN)处理可抑制IRCC小鼠的肿瘤形成，h-(NGS + TSN)处理可抑制COX-2通路。

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Protective effect of notoginsenoside and tanshinone IIA on inflammation-related colorectal cancer mice and the inhibition effect on COX-2 expression

Objective

To explore the preventive effects and possible me-chanisms of action of notoginsenoside (NGS) and tanshinone IIA (TSN) in inflammation-related colorectal cancer (IRCC) in mice.

Methods

Eighty-eight male C57BL/6 mice were randomly assigned to 11 groups (n = 8 each group). Azomethane oxide + dextran sulfate (AOM + DSS) model control (model), NGS low-dose (l-NGS), NGS medium-dose (m-NGS), NGS high-dose (h-NGS), TSN low-dose (l-TSN), TSN medium-dose (m-TSN), TSN high-dose (h-TSN), (NGS + TSN) low-dose [l-(NGS + TSN)], (NGS + TSN) medium-dose [m-(NGS + TSN)], (NGS+TSN) high-dose [h-(NGS + TSN)], and blank groups were established. The first 10 groups were intraperitoneally injected with AOM to induce inflammatory colon cancer, whereas the blank group was intraperitoneally injected with 0.9% NaCl solution. The first 10 groups drank a 2.5% sodium DSS aqueous solution continuously from day 5 for three cycles (one cycle: five days, every three weeks), and the blank group was allowed free access to water. Drug groups were administered NGS (low, medium, or high dose), TSN (low, medium, or high dose), or NGS + TSN (low, medium, or high dose), and the model and blank groups were administered saline by lavage until the end of the experiment. The general activity, body weight, and survival rate of and incidence of adenocarcinoma in mice were detected and the expression of cyclooxygenase 2 (COX-2) was detected by immunohistochemistry.

Results

(1) The survival rate of mice with IRCC in the h-NGS, m-TSN, h-TSN, m-(NGS + TSN), and h-(NGS + TSN) groups was significantly increased than that in other groups (P < 0.05). (2) The incidence of tumors in the h-(NGS + TSN), m-TSN, and l-NGS groups was significantly lower than that in the model group (P < 0.05). (3) The expression level of COX-2 in tumor tissues of mice in the m-(NGS + TSN) and h-(NGS + TSN) groups was significantly lower than that in the model group (P < 0.05).