[不同细胞培养物对苏云金芽孢杆菌耐热外毒素的相对敏感性]。

Revue canadienne de biologie Pub Date : 1978-06-01
P Laurent
{"title":"[不同细胞培养物对苏云金芽孢杆菌耐热外毒素的相对敏感性]。","authors":"P Laurent","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The \"thermostable\" B. thuringiensis exotoxin is active on cell cultures of Mammals \"in vitro\", except on the KB strain from a human tumor. The primary cultures are the most sensitive: first, with monkey kidney cells, the growth is inhibited by 0.1 mg of toxin per ml; next, the young rabbit kidney cells react to 0.25 mg of toxin per ml. The established lines of cells come last: human diploid cells (Lyon 4) and heteroploid cells (BHK21C13), with the same active dose of 1 mg of toxin per ml. No protection is obtained by adding ATP to monkey kidney cells at the same time as the exotoxin.</p>","PeriodicalId":21345,"journal":{"name":"Revue canadienne de biologie","volume":"37 2","pages":"127-30"},"PeriodicalIF":0.0000,"publicationDate":"1978-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Relative sensitivity of various cell cultures to the thermostable exotoxin of B. thuringiensis].\",\"authors\":\"P Laurent\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The \\\"thermostable\\\" B. thuringiensis exotoxin is active on cell cultures of Mammals \\\"in vitro\\\", except on the KB strain from a human tumor. The primary cultures are the most sensitive: first, with monkey kidney cells, the growth is inhibited by 0.1 mg of toxin per ml; next, the young rabbit kidney cells react to 0.25 mg of toxin per ml. The established lines of cells come last: human diploid cells (Lyon 4) and heteroploid cells (BHK21C13), with the same active dose of 1 mg of toxin per ml. No protection is obtained by adding ATP to monkey kidney cells at the same time as the exotoxin.</p>\",\"PeriodicalId\":21345,\"journal\":{\"name\":\"Revue canadienne de biologie\",\"volume\":\"37 2\",\"pages\":\"127-30\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revue canadienne de biologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revue canadienne de biologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

“耐热性”苏云金芽胞杆菌外毒素对哺乳动物体外培养的细胞有活性,但对来自人类肿瘤的KB菌株除外。原代培养是最敏感的:首先,用猴肾细胞,每ml 0.1 mg的毒素抑制生长;接下来,幼兔肾细胞对每毫升0.25毫克的毒素起反应。最后是已建立的细胞系:人二倍体细胞(Lyon 4)和异倍体细胞(BHK21C13),每毫升毒素的活性剂量相同,为1毫克。与外毒素同时添加ATP到猴肾细胞中没有得到保护。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Relative sensitivity of various cell cultures to the thermostable exotoxin of B. thuringiensis].

The "thermostable" B. thuringiensis exotoxin is active on cell cultures of Mammals "in vitro", except on the KB strain from a human tumor. The primary cultures are the most sensitive: first, with monkey kidney cells, the growth is inhibited by 0.1 mg of toxin per ml; next, the young rabbit kidney cells react to 0.25 mg of toxin per ml. The established lines of cells come last: human diploid cells (Lyon 4) and heteroploid cells (BHK21C13), with the same active dose of 1 mg of toxin per ml. No protection is obtained by adding ATP to monkey kidney cells at the same time as the exotoxin.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信