Richard E. Thompson , Edwin L.-F. Li, H. Olin Spivey, John P. Chandler, Alan J. Katz , James R. Appleman
{"title":"5-磷酸核糖基α-1-焦磷酸H+和Mg2+配合物的表观稳定性常数","authors":"Richard E. Thompson , Edwin L.-F. Li, H. Olin Spivey, John P. Chandler, Alan J. Katz , James R. Appleman","doi":"10.1016/S0006-3061(00)82004-9","DOIUrl":null,"url":null,"abstract":"<div><p>Apparent Mg<sup>2+</sup> and H<sup>+</sup> stability constants of 5-phosphoribosyl α-1-pyrophosphate (ligand, L) complexes were determined from pH titration data at 25°C with an average of 0.17 M NaCl or KCl and 0.20 M ionic strength. The logarithms of calculated macroscopic overall stability constants are: 3.2 (MgL<sup>3-</sup>), 4.8 (Mg<sub>2</sub>L<sup>-</sup>), 6.5 (HL<sup>4-</sup>), 12.4 (H<sub>2</sub>L<sup>3-</sup>), 9.4 (MgHL<sup>2-</sup>), and 11.0 (MgH<sub>2</sub>L). Comparison of the stepwise Mg<sup>2+</sup> stability constants (log <em>k</em> = 3.2 and 1.6) with those of MgADP<sup>-</sup> and MgAMP or Mg-hexose-1-P suggests that the first and second Mg<sup>2+</sup> bind to the 1-PP and 5-P groups of the ligand, respectively. Reasonable assumptions about relative microscopic constants indicate that several of the microscopic isomers do not achieve significant concentrations over a large range of conditions. Judging from other data on organophosphate complexes, it is likely that the constants of this study may be extrapolated with little error to other conditions of ionic strength 0.1–0.2 M) and temperature (e.g., 15–35°C), and widely different monovalent ion concentrations.</p></div>","PeriodicalId":9177,"journal":{"name":"Bioinorganic chemistry","volume":"9 1","pages":"Pages 35-45"},"PeriodicalIF":0.0000,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0006-3061(00)82004-9","citationCount":"13","resultStr":"{\"title\":\"Apparent stability constants of H+ and Mg2+ complexes of 5-phosphoribosyl α-1-pyrophosphate\",\"authors\":\"Richard E. Thompson , Edwin L.-F. Li, H. Olin Spivey, John P. Chandler, Alan J. Katz , James R. Appleman\",\"doi\":\"10.1016/S0006-3061(00)82004-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Apparent Mg<sup>2+</sup> and H<sup>+</sup> stability constants of 5-phosphoribosyl α-1-pyrophosphate (ligand, L) complexes were determined from pH titration data at 25°C with an average of 0.17 M NaCl or KCl and 0.20 M ionic strength. The logarithms of calculated macroscopic overall stability constants are: 3.2 (MgL<sup>3-</sup>), 4.8 (Mg<sub>2</sub>L<sup>-</sup>), 6.5 (HL<sup>4-</sup>), 12.4 (H<sub>2</sub>L<sup>3-</sup>), 9.4 (MgHL<sup>2-</sup>), and 11.0 (MgH<sub>2</sub>L). Comparison of the stepwise Mg<sup>2+</sup> stability constants (log <em>k</em> = 3.2 and 1.6) with those of MgADP<sup>-</sup> and MgAMP or Mg-hexose-1-P suggests that the first and second Mg<sup>2+</sup> bind to the 1-PP and 5-P groups of the ligand, respectively. Reasonable assumptions about relative microscopic constants indicate that several of the microscopic isomers do not achieve significant concentrations over a large range of conditions. Judging from other data on organophosphate complexes, it is likely that the constants of this study may be extrapolated with little error to other conditions of ionic strength 0.1–0.2 M) and temperature (e.g., 15–35°C), and widely different monovalent ion concentrations.</p></div>\",\"PeriodicalId\":9177,\"journal\":{\"name\":\"Bioinorganic chemistry\",\"volume\":\"9 1\",\"pages\":\"Pages 35-45\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0006-3061(00)82004-9\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioinorganic chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0006306100820049\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioinorganic chemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0006306100820049","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Apparent stability constants of H+ and Mg2+ complexes of 5-phosphoribosyl α-1-pyrophosphate
Apparent Mg2+ and H+ stability constants of 5-phosphoribosyl α-1-pyrophosphate (ligand, L) complexes were determined from pH titration data at 25°C with an average of 0.17 M NaCl or KCl and 0.20 M ionic strength. The logarithms of calculated macroscopic overall stability constants are: 3.2 (MgL3-), 4.8 (Mg2L-), 6.5 (HL4-), 12.4 (H2L3-), 9.4 (MgHL2-), and 11.0 (MgH2L). Comparison of the stepwise Mg2+ stability constants (log k = 3.2 and 1.6) with those of MgADP- and MgAMP or Mg-hexose-1-P suggests that the first and second Mg2+ bind to the 1-PP and 5-P groups of the ligand, respectively. Reasonable assumptions about relative microscopic constants indicate that several of the microscopic isomers do not achieve significant concentrations over a large range of conditions. Judging from other data on organophosphate complexes, it is likely that the constants of this study may be extrapolated with little error to other conditions of ionic strength 0.1–0.2 M) and temperature (e.g., 15–35°C), and widely different monovalent ion concentrations.