{"title":"hemin介导的高效液相色谱法测定褪黑素","authors":"H. Iizuka, K. Someya, T. Yajima","doi":"10.1016/j.ics.2007.07.047","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>A new fluorometric method for the determination of </span>melatonin<span> using a reversed-phase high-performance liquid chromatography was developed. Melatonin, by heating in the presence of hemin<span><span> and sodium percarbonate<span> at 100 °C for 15 min, gave a strong fluorescent product (Ex. 247 nm, Em. 384 nm). Employing a solid phase extraction prior to </span></span>HPLC, the level of linear range reached to between 5 to 100 pmol/l (</span></span></span><em>R</em><sup>2</sup> <!-->=<!--> <!-->0.9906).</p></div>","PeriodicalId":84918,"journal":{"name":"International congress series","volume":"1304 ","pages":"Pages 409-414"},"PeriodicalIF":0.0000,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ics.2007.07.047","citationCount":"6","resultStr":"{\"title\":\"Hemin-mediated fluorometric determination of melatonin by high-performance liquid chromatography\",\"authors\":\"H. Iizuka, K. Someya, T. Yajima\",\"doi\":\"10.1016/j.ics.2007.07.047\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>A new fluorometric method for the determination of </span>melatonin<span> using a reversed-phase high-performance liquid chromatography was developed. Melatonin, by heating in the presence of hemin<span><span> and sodium percarbonate<span> at 100 °C for 15 min, gave a strong fluorescent product (Ex. 247 nm, Em. 384 nm). Employing a solid phase extraction prior to </span></span>HPLC, the level of linear range reached to between 5 to 100 pmol/l (</span></span></span><em>R</em><sup>2</sup> <!-->=<!--> <!-->0.9906).</p></div>\",\"PeriodicalId\":84918,\"journal\":{\"name\":\"International congress series\",\"volume\":\"1304 \",\"pages\":\"Pages 409-414\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.ics.2007.07.047\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International congress series\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0531513107004554\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International congress series","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0531513107004554","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Hemin-mediated fluorometric determination of melatonin by high-performance liquid chromatography
A new fluorometric method for the determination of melatonin using a reversed-phase high-performance liquid chromatography was developed. Melatonin, by heating in the presence of hemin and sodium percarbonate at 100 °C for 15 min, gave a strong fluorescent product (Ex. 247 nm, Em. 384 nm). Employing a solid phase extraction prior to HPLC, the level of linear range reached to between 5 to 100 pmol/l (R2 = 0.9906).
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