Ji Su Lee, Seon Mi Lee, Hyung Seok Son, Yi Na Yoon, Jung Eun Shin, Woo Jun Sul, Da Ae Yu, Yong Beom Choe, Yang Won Lee
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引用次数: 3
摘要
背景:近年来，微生物组研究在不同皮肤区域得到积极开展。然而，目前还没有研究将健康个体和慢性外耳炎患者耳道内的细菌和真菌微生物群进行比较。目的:探讨健康人群与慢性外耳炎患者耳道样品中真菌和细菌微生物群落分布的差异。方法:选取24例双侧慢性外耳炎患者和24例健康对照者，采用棉签法采集双侧耳道标本。为了表征真菌和细菌群落，我们分别对16S rRNA V4-V5和ITS1区域进行了测序和分析，使用了Quantitative Insights into Microbial Ecology 2。结果:细菌和真菌的α多样性分析证实，患者组的丰富度和均匀度均有所下降。细菌多样性分析证实，这些参数在对照组和患者组之间存在差异。真菌的多样性分析显示各组之间没有差异。结论:慢性外耳炎患者的皮肤微生物群与健康人不同。
Analysis of the Microbiome of the Ear Canal in Normal Individuals and Patients with Chronic Otitis Externa.
Background: Recently, microbiome research has been actively conducted for various skin areas. However, no study has yet compared the microbiome of bacteria and fungi in the ear canal of healthy individuals and patients with chronic otitis externa in Korea.
Objective: This study aimed to investigate the difference in the distribution of fungal and bacterial microbial communities in ear canal samples of healthy individuals and patients with chronic otitis externa.
Methods: In 24 patients with bilateral chronic otitis externa and 24 healthy controls, cotton swabs were used to obtain samples from the bilateral ear canal. To characterize the fungal and bacterial communities, we sequenced and analyzed the 16S rRNA V4-V5 and ITS1 regions using Quantitative Insights into Microbial Ecology 2, respectively.
Results: The alpha diversity analysis for bacteria and fungi confirmed that both richness and evenness decreased in the patient group. The beta diversity analysis for bacteria confirmed that these parameters differed between the control and patient groups. The beta diversity analysis for fungi showed no difference between the groups.
Conclusion: We observed different skin microbiomes in the patients with chronic otitis externa compared with those in the healthy individuals.
期刊介绍：
Annals of Dermatology (Ann Dermatol) is the official peer-reviewed publication of the Korean Dermatological Association and the Korean Society for Investigative Dermatology. Since 1989, Ann Dermatol has contributed as a platform for communicating the latest research outcome and recent trend of dermatology in Korea and all over the world.
Ann Dermatol seeks for ameliorated understanding of skin and skin-related disease for clinicians and researchers. Ann Dermatol deals with diverse skin-related topics from laboratory investigations to clinical outcomes and invites review articles, original articles, case reports, brief reports and items of correspondence. Ann Dermatol is interested in contributions from all countries in which good and advanced research is carried out. Ann Dermatol willingly recruits well-organized and significant manuscripts with proper scope throughout the world.
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