组织蛋白质组学和磷酸化蛋白质组学分析预测结直肠癌复发。

IF 3.8 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Expert Review of Proteomics Pub Date : 2022-04-01 DOI:10.1080/14789450.2022.2142566

Liyun Ji, Zeyuan Wang, Yin Ji, Huiyu Wang, Miao Guo, Lu Zhang, Peng Wang, Hua Xiao

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引用次数: 0

摘要

背景:许多II/III期结直肠癌(CRC)患者在常规治疗后可能复发。异常磷酸化可以调节肿瘤的病理生理过程，发现特异性磷酸化蛋白是预测结直肠癌复发的有效途径。研究设计和方法:比较复发组(n = 5)和非复发组(n = 5) II/III期CRC患者的组织蛋白质组和磷酸化蛋白质组。磷酸肽富集Ti4+-IMAC物质。我们利用无标记的基于定量的蛋白质组学来筛选两组之间差异表达的蛋白质和磷酸肽。生物信息学分析采用基因本体(GO)分析和独创性途径分析(IPA)。结果:复发组(z评分-2.229)的免疫应答相对于非复发组(z评分1.982)差，而复发组肿瘤活力更活跃(z评分2.895)，可能导致复发风险增加。三个磷酸化位点(TP53BP1的1362磷酸化位点、VCL的809磷酸化位点和STK10的438磷酸化位点)的磷酸化程度可能是可靠的预后生物标志物。结论:在术后随访中发现了一些有希望预测复发风险的蛋白和磷酸肽。

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Proteomics and phosphoproteomics analysis of tissues for the reoccurrence prediction of colorectal cancer.

Background: Many stage II/III colorectal cancer (CRC) patients may relapse after routine treatments. Aberrant phosphorylation can regulate pathophysiological processes of tumors, and finding characteristic protein phosphorylation is an efficient approach for the prediction of CRC relapse.

Research design and methods: We compared the tissue proteome and phosphoproteome of stage II/III CRC patients between the relapsed group (n = 5) and the non-relapsed group (n = 5). Phosphopeptides were enriched with Ti⁴⁺-IMAC material. We utilized label-free quantification-based proteomics to screen differentially expressed proteins and phosphopeptides between the two groups. Gene Ontology (GO) analysis and Ingenuity Pathway Analysis (IPA) were used for bioinformatics analysis.

Results: The immune response of the relapsed group (Z-score -2.229) was relatively poorer than that of the non-relapsed group (Z-score 1.982), while viability of tumor was more activated (Z-score 2.895) in the relapsed group, which might cause increased relapse risk. The phosphorylation degrees of three phosphosites (phosphosite 1362 of TP53BP1, phosphosite 809 of VCL and phosphosite 438 of STK10) might be reliable prognostic biomarkers.

Conclusions: Some promising proteins and phosphopeptides were discovered to predict the relapse risk in postoperative follow-ups.

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来源期刊

Expert Review of Proteomics 生物-生化研究方法

CiteScore

7.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Expert Review of Proteomics (ISSN 1478-9450) seeks to collect together technologies, methods and discoveries from the field of proteomics to advance scientific understanding of the many varied roles protein expression plays in human health and disease. The journal coverage includes, but is not limited to, overviews of specific technological advances in the development of protein arrays, interaction maps, data archives and biological assays, performance of new technologies and prospects for future drug discovery. The journal adopts the unique Expert Review article format, offering a complete overview of current thinking in a key technology area, research or clinical practice, augmented by the following sections: Expert Opinion - a personal view on the most effective or promising strategies and a clear perspective of future prospects within a realistic timescale Article highlights - an executive summary cutting to the author''s most critical points.